藏绵羊JNK1基因遗传特征及其适应性进化分析  

Genetic Characteristics and Adaptive Evolution Analysis of JNK1 Gene in Tibetan Sheep

作　　者：刘宪 刘秀 王继卿 李少斌 胡江 罗玉柱 Liu Xian ,Liu Xiu ,Wang Jiqing ,Li Shaobin ,Hu Jiang, Luo Yuzhu(Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Gansu, 73007)

机构地区：[1]甘肃农业大学动物科学技术学院甘肃省草食动物生物技术重点实验室

出　　处：《基因组学与应用生物学》2018年第3期1152-1159,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31260546);国家科技支撑计划(2012BAD13B05);甘肃省自然科学基金(1606RJZA074);甘肃省农业生物技术研发项目(GNSW-2016-24)联合资助

摘　　要：JNKs（c—Jun N—terminal kinases）是丝裂原活化蛋白激酶（MAPK）家族中的成员，主要参与动物应急反应和组织发育等生命活动的调控过程。为分析藏绵羊在高寒低氧环境中的适应性进化机制，利用PCR－SSCP技术对藏绵羊、甘肃高山细毛羊和滩羊的JNK1基因第1、第8外显子的分子遗传特征进行分析，从而明确3个绵羊群体JNK1基因遗传序列特征、单核苷酸突变位点、单倍型组成和群体遗传特性。结果表明，在3个绵羊群体JNK1基因第1外显子区均检测到AA、BB和AB3个基因型和1个突变位点，其中AA基因型和A等位基因为藏绵羊的优势基因型和优势等位基因，显著高于甘肃高山细毛羊和滩羊。在第8外显子区检测到EE、FF和EF3个基因型，发现3个碱基插入和1个SNPs，其中等位基因F为藏绵羊和甘肃高山细毛羊的优势等位基因。研究结果与3个绵羊群体所处海拔高度及所受外界环境应激反应（高寒低氧）的强弱是相一致的，表明JNK1基因可以作为藏绵羊的高原适应性进化研究以及抗逆／病性分子选育的候选基因。研究结果将为藏绵羊的遗传改良和种质创新提供基础数据。JNKs （c-Jun N-terminal kinases） is a member ofmitogen activated protein kinase （MAPK） family and it mainly participates in animal emergency response and the process of regulating the development of life activities and so on. In order to analyze the adaptive evolution mechanism of Tibetan sheep in alpine and hypoxic environment, molecular genetic characteristics of JNK1 gene exons 1 and exons 8 were analyzed using PCR-SSCP technology respectively in Tibetan sheep, Gansu alpine Merion and Tan sheep so as to identify the gene sequence, single nucleotide mutations （SNP）, haploid type composition and population genetic characteristics ofJNK1 gene in three sheep populations. Three genotypes ofAA, BB and AB and 1 mutation site were detected in the first exon region of JNK1 gene in three sheep populations, in which AA genotype and A allele were the dominant genotypes and dominant alleles of Tibetan sheep, which were significantly higher than those of Gansu alpine fine wool sheep and Tan sheep. In the 8th exon region, 3 genotypes of EE, FF and EF were detected, and 3 bases insertand 1 SNPs were found, which meant that allele F was the dominant allele of Tibetan sheep and Gansu alpine fine wool sheep. The results were consistent with the altitude of the three sheep populations and the intensity of the stress response to the external environment （alpine-cold and low oxygen）, which pointed that JNK1 gene could be used as a candidate gene on study of adaptation evolution of Tibetan sheep and selection of stress resistance molecular. The results might provide the basic data for genetic improvement and germplasm innovation of Tibetan sheep.

关 键 词：JNK1基因 PCR-SSCP 遗传特征 绵羊 

分 类 号：S826[农业科学—畜牧学]