广西南丹瑶鸡PGC-1α基因646位点多态性分析  

Polymorphisms Analysis of PGC-1α in 646 Locus of Guangxi Nandan-Yao Chicken

作　　者：邓继贤[2] 蒋世强[1] 陈秋明[1] 杨祝良[1] 夏利[1] 黎卓炎 蒋和生[1] 杨秀荣[1] Deng Jixian 2 ,Jiang Shiqiang 1 ,Chen Qiuming 1, Yang Zhuliang1, Xia Li 1, Li Zhuoyan 3 ,Jiang Hesheng 1, Yang Xiurong 1(1 Collage of Animal Science and Technology, Guangxi University, Nanning, 530004; 2 Guangxi Institute of Animal Sciences, Nanning, 530001; 3 Guangxi Guigang Gangfeng Agriculture and Animal Husbandry Co., LTD, Guigang, 53711)

机构地区：[1]广西大学动物科学技术学院,南宁530004 [2]广西壮族自治区畜牧研究所,南宁530001 [3]广西贵港市港丰农牧有限公司,贵港537113

出　　处：《基因组学与应用生物学》2018年第3期1160-1162,共3页Genomics and Applied Biology

基　　金：广西创新驱动发展专项资金项目(桂科AA17204027);国家自然科学基金地区基金项目(31660635);广西水产畜牧科技推广应用项目(桂渔牧科201701;桂渔牧科201633008;桂渔牧科201633025)基金共同资助

摘　　要：广西南丹瑶鸡是广西优良的地方品种，其特点是皮脂薄且肉质好，但其形成的分子机制不清楚。过氧化物酶体增殖物激活受体γ辅激活子1α（peroxisome proliferator-activated receptor-γ coactivator-1, PGC-1α）是影响脂肪沉积的重要候选基因。本研究以广西麻鸡作为对照，利用PCR-RFLP技术分析了广西南丹瑶鸡PGC-1α基因646位点的多态性。结果显示，广西南丹瑶鸡和广西麻鸡PGC-1α基因646位点都存在A和G两个等位基因，A等位基因频率在广西南丹瑶鸡和广西麻鸡分别为0．35和0．23。AA、AG和GG3种基因型频率在广西南丹瑶鸡中分别为0．05、0．60和0．35，在广西麻鸡分别为0．07、0．33和0．6。本研究的结果提示A等位基因可能有助于减少广西南丹瑶鸡皮下脂肪的沉积。Guangxi Nandan-Yao chicken is excellent local breeding, and is characterized by thin leather fat and good meat quality. However it is not clear on the its molecular mechanism. Peroxisome proliferator-activated receptor-γ coactivator-1 （PGC-1α） is one of important candidate genes affecting the fat deposition. The objective of current study was to analyze the polymorphisms of PGC-1α in 646 Locus of Guangxi Nandan-Yao employing the PCR-RFLP technology. Guangxi partridge was taken as the control breeding. The result showed that there were A and G two alleles in the PGC-1α locus in Nandan-Yao and Guangxi partridge. The frequency of A alleles was 0.35 and 0.23 in Nandan-Yao and Guangxi partridge, respectively. The frequency of AA, AG, and GG genotypes was 0.05, 0.60 and 0.35 in Nandan-Yao, respectively, while was 0.07, 0.33 and 0.6 in Guangxi partridge, respectively. The current study indicated A allele might be benificial to reducing the skin fat deposition in Guangxi Nandan-Yao.

关 键 词：南丹瑶鸡 脂肪沉积 PGC—1α 646位点 

分 类 号：S831[农业科学—畜牧学]