转基因水稻U41三引物检测方法的建立  

Establishment of Three Primer Detection Method for Transgenic Rice U41

作　　者：张斌[1] 阮颖[1] Zhang Bin ,Ruan Ying(Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 41012)

机构地区：[1]湖南农业大学生物科学技术学院湖南省作物种质资源创新和利用重点实验室

出　　处：《基因组学与应用生物学》2018年第3期1308-1314,共7页Genomics and Applied Biology

基　　金：湖南省研究生科研创新项目(CX2015B239)资助

摘　　要：对于研究转基因农作物来说，建立其检测方法至关重要。本研究利用TAIL-PCR法，获得了转基因水稻U41的右旁侧序列，结果显示T—DNA插入位点在水稻1号染色体第22013～22014之间。通过设计引物扩增左旁侧序列，建立了U41转化事件特异性检测的方法，可以从含0．1％目的基因中扩增出529bp的特异性条带；还建立了三引物检测的方法，能快速和准确地鉴定U41的纯合株系。这些方法的建立，缩短了获得U41纯合体的时间，对U41的检测、研究和利用具有实用价值。It is of vital importance to establish a detection method for transgenic crops research. This study obtained the right flanking sequences of transgenic rice U41 by TAIL-PCR method, and revealed that the T-DNA insertion site was between 22 013^rd and 22 014^th in rice chromosome 1. Moreover, this study obtained the lett flanking sequences by designing primers, established an event-specific detection method for U41 transformation which could amplify a 529 bp specific band from 0.1% target genes, and set up a detection method with three primers which could identify the homozygous lines of U41 rapidly and accurately. The establishment of two methods shortened the time of obtaining U41 homozygote, and might have practical values for the detection, research and utilization of U41.

关 键 词：纯合子鉴定 转基因水稻 三引物检测法 旁侧序列 事件特异性检测 

分 类 号：S511[农业科学—作物学]