干旱胁迫对日本荚蒾幼苗光合生理特性的影响  

Effects of drought stress on the photosynthetic characteristics of Viburnum japonicum seedlings

作　　者：李瑞姣 陈献志 岳春雷[3] 李贺鹏[3] 王珺[3] 郭亮 杨乐[3] LI Ruijiao1, CHEN Xianzhi2, YUE Chunlei3 , LI Hepeng3, WANG Jun3, GUO Liang4, YANG Le3(1 School of Forestry and Bio-technology, Zhejiang Agricultural ＆ Forestry University, Lin＇an 311300, China ;2 Linhai Forestry Technology Extension Station of Zhejiang, Linhai 317000, China ;3 Zhejiang Forestry Academy, Hangzhou 310000, China ;4 Taizhou Jiaojiang Agroforestry Bureau of Zhejiang, Taizhou 318000, Chin)

机构地区：[1]浙江农林大学林业与生物技术学院,临安311300 [2]浙江省临海市林业技术推广总站,临海317000 [3]浙江省林业科学研究院,杭州310000 [4]浙江省台州市椒江区农林局,台州318000

出　　处：《生态学报》2018年第6期2041-2047,共7页Acta Ecologica Sinica

基　　金：浙江省省院合作项目(2015SY01);浙江省院所专项项目(2016F30011)

摘　　要：为了探讨极小种群野生植物日本荚蒾(Viburnum japonicum)对干旱胁迫的响应,以日本荚蒾二年实生苗为材料进行盆栽控水实验,设置正常供水(CK)、轻度干旱(LS)、中度干旱(MS)和重度干旱(SS)4个水分处理,研究持续干旱处理对日本荚蒾幼苗光合生理特性的影响。结果表明:在CK、LS、MS水分梯度下,日本荚蒾幼苗生长正常,但在SS胁迫下受影响明显,植株全部死亡。各胁迫阶段叶片净光合速率(P_n)、SPAD值、最大净光合速率(P_(nmax))、表观量子效率(AQY)、暗呼吸速率(R_d)表现出随水分梯度的降低而减小,光饱和点(LSP)、光补偿点(LCP)则相反,各水分梯度下AQY随时间延长逐渐上升,SS处理幼苗在胁迫45d及以后P_n均为负值。最大光化学效率(F_v/F_m)在胁迫过程中变化范围较小;实际光量子效率(ΦPSII)、电子传递速率(ETR)、光化学猝灭系数(qP)随土壤水分的减少而降低,非光化学猝灭系数(NPQ)逐渐上升,LS、MS与CK间变化幅度较小,但SS与CK间差距逐渐增大。结果表明在轻度和中度干旱下日本荚蒾幼苗表现出一定的适应性和耐干旱能力,而长期重度干旱则对幼苗影响较为严重。Viburnum japonicum is an evergreen plant species,only found in the islands of Taizhou and Zhoushan,Zhejiang Province in China. This species was listed in the Directory of Plant Species with Extremely Small Populations in Zhejiang Province,because the wild individuals were extremely rare. To study its resistance mechanism and adaptation to drought,reveal the cause endangering the species,2-year-old seedlings of V. japonicum were potted and grown under four soil water conditions： normal water supply（ CK,80% of the field moisture capacity）,mild water stress（ LS,60% of the field moisture capacity）,moderate water stress（ MS,40% of the field moisture capacity）,and severe water stress（ SS,20% of the field moisture capacity）. Determinations were implemented on photosynthetic characteristics in the 15,30,45,60,and 75 d of treated seedlings. The result demonstrated that V. japonicum seedlings could grow normally under CK,LS,and MS,but they died at 75 d under SS. The net photosynthetic rate（ Pn）,SPAD value,maximum net photosynthetic rate（Pnmax）,apparent quantum yield（ AQY）,and dark respiration rate（ Rd） of different treated seedling leaves decreased with the decrease of soil water content,while the light saturation point（ LSP） and light compensation point（ LCP） increased. The AQY increased gradually over time under all moisture gradients,and Pn was below 0μmol m-2 s-1 from 45 d under SS. The changes in the maximum quantum yield of photosystem II（ PSII）（ Fv/Fm） was small during any stress; the actual photochemical efficiency of PSII（ Φ（PSII））,electron transport rate（ETR） and photochemical quenching coefficient（qP） of seedlings decreased with increasing water stress,but the non-photochemical quenching coefficient（ NPQ） showed the opposite trend. The differences in the chlorophyll fluorescence parameters changed slightly among LS,MS,and CK,while the gaps between SS and CK increased. The experiment resulted that V. japonicum seedlings under LS and MS showed

关 键 词：日本荚蒾 干旱胁迫 光合参数 叶绿素荧光参数 生理特性 

分 类 号：Q945.78[生物学—植物学]