多效唑和干旱胁迫对毛竹实生苗活力、光合能力及非结构性碳水化合物的影响  

Effects of PP_(333) and drought stress on the activity,photosynthetic characteristics,and non-structural carbohydrates of Phyllostachys edulis seedlings

作　　者：杨丽芝 潘春霞 邵珊璐 陶晨悦 王威 应叶青[1] YANG Lizhi1 , PAN Chunxia2, SHAO Shanlu1 , TAO Chenyue1 , WANG Wei1 , YING Yeqing1(1 State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin＇an 311300, China 2 Forestry Technology Extension Center of Anji District, Huzhou 313300, Chin)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,临安311300 [2]安吉县林业技术推广中心,湖州313300

出　　处：《生态学报》2018年第6期2082-2091,共10页Acta Ecologica Sinica

基　　金："十三五"国家重大研发计划课题(2016YFD0600901)

摘　　要：以1年生毛竹实生苗为研究对象,研究多效唑对不同水分条件下毛竹实生苗的叶绿素含量、光合参数、非结构性碳水化合物(NSC)含量、碳氮比、根系活力的影响。设置3个水分梯度:W1(75%相对田间持水量,CK)、W2(50%相对田间持水量,中度干旱)和W3(35%相对田间持水量,重度干旱),以及2个多效唑浓度:P1(0mg/L)、P2(40mg/L)。结果表明:随干旱强度增加,P1W1、P1W2、P1W3处理叶色逐渐变淡。与对照P1W1相比,P1W2和P1W3处理下叶绿素a、叶绿素b、类胡萝卜素、叶绿素a/b和叶绿素总含量显著下降(P<0.05),Pn、Tr、WUE显著下降(P<0.05),Ls显著上升(P<0.05),毛竹叶片及根系中非结构性碳水化合物(NSC)含量显著上升(P<0.05),毛竹根系活力显著下降。多效唑处理后,P2W2和P2W3的叶片色素含量相对于P2W1显著提高,但P2W2与P2W3无显著差异。同时,施加多效唑使Pn显著提高,P2W3较P1W3增加了146.9%。此外,P2W3处理使可溶性糖大量积累,达最大值3.41mg/g;毛竹叶片及根系淀粉含量显著上升,根系活力显著提高。试验揭示了多效唑通过提高干旱水平下毛竹实生苗的根系活力、光合速率,增加光合色素、非结构性碳水化合物含量,并将养分从地上转移到地下部分,进而抵御干旱胁迫带来的伤害。We investigated the effects of paclobutrazol（ PP333） on the chlorophyll,photosynthetic parameters,nonstructural carbohydrates（ NSC）,C/N ratio,root activity on one-year-old phyllostachys edulis seedlings. Experiments were degined with three water treatments（ W1： well-watered with 75% field capacity; W2： water stress with 50% FC; W3 water stress with 35% FC） and two levels of PP333 treatments（ P1： 0 mg/L; P2： 40 mg/L）. Results showed that： as the drought increased,leaf color of P. edulis seedlings gradually faded and the concentration of Chla,Chlb,Car,Chla/b and total chl significantly decreased（ P0.05）,Pn,Tr and WUE significantly decreased（ P〈0. 05）,Ls significantly increased（ P〈0.05）,the NSC content in the leaf and root significantly increased（ P〈0.05）,root activity significantly decreased（ P〈0.05）. When PP333 was applied,the chlorophyll content were significantly increased under both P2W2 and P2W3 treatments relative to P2W1 treatment,but no significant difference was found between P2W2 and P2W3. Also,P2W3 significantly increased Pn by 146. 9% compared with P1W3 treatment,and the total soluble sugar content significantly increased up to 3.41 mg/g. Besides,P2W3 significantly increased the total soluble sugar content and the starch content in leaf and root,and root： shoot ratio was significantly increased. Our study demonstrated that PP_（333） application could effectively improve the drought adaptability of P. edulis seedlings by increasing the root activity and root： shoot ratio,reducing water loss and NSC content,and improving the photosynthetic pigments and photosynthetic capacity.

关 键 词：干旱 多效唑 毛竹 根系活力 光合能力 非结构性碳水化合物(NSC) 

分 类 号：S795.7[农业科学—林木遗传育种]