爪哇黄芩二氢黄酮对HepG-2细胞增殖的影响  

Effects of flavonone in Scutellaria javanica Jungh. on HepG-2 cells proliferation

作　　者：于蕾[1,2,3] 姜波 王鑫[1,2,3] 杨经辉 YU Lei1,2,3,JIANG Bo1,2,3,WANG Xin1,2,3, YANG Jing-hui1,2,3(1. Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China; 2. Institute of Materia Medica and Postdoctoral Programme of Harbin University of Commerce, Harbin 150076, China; 3. Engineering Reseach Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076,China)

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]抗肿瘤天然药物教育部工程研究中心,哈尔滨150076 [3]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2018年第2期129-133,158,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省普通高等学校肿瘤预防与抗肿瘤药物研究重点实验室开放课题(CPAT-2012003);哈尔滨市科技局创新人才项目(2014RFQXJ154);哈尔滨商业大学博士科研启动项目(12DL008);哈尔滨商业大学科研团队计划(项目号:2016TD002);哈尔滨商业大学青年创新人才支持项目(2016QN057)

摘　　要：为研究爪哇黄芩二氢黄酮活性成分的抗肿瘤作用,筛选了爪哇黄芩活性部位和敏感细胞株,并研究了爪哇黄芩活性二氢黄酮对敏感细胞株HepG-2细胞增殖的影响,为开发天然药物奠定了实验基础.选取体外培养的人肝癌细胞HepG-2、胃癌细胞SGC-7901、肺癌细胞A549、乳腺癌细胞MCF-7及神经胶质瘤细胞C6为模型,以半数抑制浓度(IC50)为指标,采用MTT法测定爪哇黄芩石油醚、二氯甲烷、正丁醇提取部位对5种肿瘤细胞的增殖抑制作用,结果表明,爪哇黄芩二氯甲烷部位为其活性部位,人肝癌细胞HepG-2为其敏感细胞株.然后,采用MTT法测定活性部位分离的二氢黄酮单体对HepG-2的抑制作用,进行活性单体的筛选.结果显示,爪哇黄芩5,6,2'-三羟基-7-甲氧基二氢黄酮IC_(50)为110.3μmol/L,爪哇黄芩5-羟基-6,7,2',6'-四甲氧基二氢黄酮IC_(50)为747.21μmol/L.实验数据表明,爪哇黄芩5,6,2'-三羟基-7-甲氧基二氢黄酮为爪哇黄芩活性二氢黄酮单体成分.To study the anti - tumor activities effects of flavanone in Scutellaria javanica Jungh. , active fraction and sensitive cell line were selected. The effect on HepG - 2 cell proliferation was researched to reveal the anti - tumor activities of flavanone in Scutellaria ja-vanica Jungh. This paper provided the experimental foundation for develop natural drugs.The human liver cancer cell HepG - 2, gastric cancer cell SGC - 7901, lung cancer cell A549, breast cancer cell MCF -7 and C6 glioma cells, cultivated in vitro, were used as models in the paper. With median inhibitory concentration （IC50） used as index,MTT col-orimetry was applied to ascertain the anti - proliferative effects of petroleum ether, dichlo- romethane and n - butyl alcohol extraction fractions in Scutellaria javanica Jungh. on the a-bove - mentioned 5 cancer cells. The dichloromethane extracted fraction of Scutellaria javan-ica Jungh. was its active fraction. HepG -2 cells is its sensitive cell line. The MTT colorimetry was adopted to ascertain the inhibiting effects of flavanone monomers extracted from the active fractions on human liver cancer cell HepG -2 to screen active monomers. The results suggested that the 5,6,2r - trihydroxy - 7 - methoxy flavanone IC50 of Scutellaria javanica Jungh. was 110. 3 μmol/L, and the 5 - hydroxyl -6,7,2,,6, - tetramethyl flavanone IC50 was 747.21 μmol/L. It was discovered that the 5 , 6 , 2＇ - trihydroxy - 7 - methoxy flavanone was the active composition of Scutellaria javanica Jungh. on human liver cancer cell HepG - 2 .

关 键 词：爪哇黄芩 二氢黄酮 人肝癌细胞HEPG-2 细胞增殖 抑制作用 

分 类 号：R285[医药卫生—中药学]