烟草慢阴离子通道蛋白基因NtSLAH1的克隆及表达分析  

Cloning and expression analysis of slowly activating anion channel gene NtSLAH1 from Nicotiana tabacum

作　　者：张慧[1] 金立锋[1] 徐国云[1] 翟妞[1] 刘萍萍[1] 陈千思[1] 郑庆霞[1] 陈霞[1] 周会娜[1] ZHANG Hui, JIN Lifeng, XU Guoyun, ZHAI Niu, LIU Pingping, CHEN Qiansi, ZHENG Qingxia, CHEN Xia, ZHOU Huina, Zhengzhou(Tobacco Research Institute of CNTC, Zhengzhou 450001, Chin)

机构地区：[1]中国烟草总公司郑州烟草研究院

出　　处：《烟草科技》2018年第3期1-6,共6页Tobacco Science & Technology

基　　金：中国烟草总公司郑州烟草研究院院长科技发展基金项目"烟草氯离子吸收转运相关基因的筛选及鉴定"(902016CA0190)

摘　　要：为了揭示烟草慢阴离子通道蛋白(Slowly activating Anion Channel,SLAC)家族成员在氯离子转运过程中的作用,通过同源克隆的方法从栽培烟草K326中克隆到1个SLAC同源基因(SLAC Homologue),命名为NtSLAH1,其开放阅读框长度为1107 bp,编码368个氨基酸,表达蛋白定位在细胞质膜上。与已报道的其他植物SLAH相比,NtSLAH1与拟南芥SLAH1和SLAH4序列相似性更高、进化距离更近。实时定量荧光PCR(qRT-PCR)结果显示,NtSLAH1在根中的表达量最高,高浓度NaCl处理下其表达量下降,表明NtSLAH1可能在烟草盐胁迫响应及氯离子吸收过程中发挥重要作用。To clarify the function of Nt SLAC（Slowly activating anion channel, SLAC） genes in chloride transport, a SLAH（SLAC homologue） gene was cloned from Nicotiana tabacum by homology search, named as NtSLAH1. The open reading frame of NtSLAH1 was 1107 bp in length and encoded 368 amino acids. The NtSLAH1 protein was located in the cytoplasmic membrane. Comparing to SLAH genes in the other plants,deduced protein sequences of NtSLAH1, AtSLAH1 and AtSLAH4 were highly similar, and NtSLAH1 was closer to AtSLAH1 and AtSLAH4 in evolutionary distance. qRT-PCR results showed that NtSLAH1 was highly expressed in root, and the expression of NtSLAH1, especially in roots, was down-regulated under salt stress. The results indicated that NtSLAH1 might play an important role in response to salt and the absorption of chloride in tobacco.

关 键 词：烟草 慢阴离子通道蛋白 NtSLAH1 氯离子 盐胁迫 

分 类 号：TS413[农业科学—烟草工业]