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作 者:李锐[1] 白建荣[1,2,3] 王秀红 张丛卓[1] 张效梅[3] 闫蕾 杨瑞娟[1] Li Rui1, Bai Jianrong1,2,3, Wang Xiuhong2, Zhang Congzhuo1, Zhang Xiaomei3, Yan Lei1,Yang Ruijuan1(1Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China; 2Modem Agricultural Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China 3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, Shanxi, Chin)
机构地区:[1]山西省农业科学院作物科学研究所,山西太原030031 [2]山西省农业科学院现代农业研究中心,山西太原030031 [3]农业部黄土高原作物基因资源与种质创新重点实验室,山西太原030031
出 处:《作物杂志》2018年第2期17-24,共8页Crops
基 金:山西省财政支农项目(2014ZYFZ-12);山西省财政支农项目(2015ZYFZ-12)
摘 要:利用SSR技术对来源于144份甜玉米群体的40个位点进行了分析,共检测出343个等位变异,每对引物检测出4~17个等位变异,平均8.58个。40个位点的多态性信息量PIC值变化范围在0.46~0.90,平均0.76/位点。标记索引指数MI变化范围在2.30~15.19,平均6.80/位点。在33个位点共检测到稀有等位变异70个,在15个位点共检测到特有等位变异16个。在144份材料中,117份材料有稀有等位变异,其中1份材料有8个稀有等位变异;14份材料有特有等位变异,2份材料有2个特有等位变异。聚类分析可初步将144份甜玉米群体划为7个类群,其中最大的Ⅰ群包括89份,第Ⅱ群包括35份。从研究结果得出群体材料的利用价值,对新的群体的合成及杂优模式的建立进行了探讨,为育种者提供新的思路和方法。Genetic diversity of 144 sweet maize populations were analyzed with 40 SSR loci. Totally 343 alleles in 144 populations were detected with an average of 6.8 ranged from 4 to 17 per locus. PIC among them ranged from 0.46 to 0.90 per locus with an average of 0.76. MI among them ranged from 2.30 to 15.19 per locus with an average of 6.80. Also 70 rare alleles from 33 loci and 16 unique alleles from 15 loci were identified. There were rare alleles in 117 populations and unique alleles in 14 populations. They were clustered into 7 groups,among which the biggest group included 89 populations and the second biggest group had 35 populations. It also provided the application values of them and new methods for new populations and heterosis utilizing models for breeders.
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