不同浓度的激素组合和有机物组合对甘薯脱毒苗快繁的影响  

Effects of Different Concentration Combinations of Hormone and Organics on Rapid Propagation of Sweet Potato Virus-Free Seedling

作　　者：闫明明[1] 陈秋骏 唐敏 刘志文[1] Yan Mingming, Chen Qiujun, Tang Min, Liu Zhiwen(School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, Chin)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《作物杂志》2018年第2期68-72,共5页Crops

基　　金：辽宁省自然科学基金(2015020682)

摘　　要：以甘薯一号脱毒试管苗为材料,研究了在MS基本培养基中添加不同浓度激素组合和有机物组合对其生长和快繁的影响。结果表明:各激素及有机物对甘薯脱毒苗生长指标总值的作用为6-BA>NAA>IAA和烟酸>肌醇>VB_1>甘氨酸>VB_6。激素及有机物最佳浓度组合分别为NAA(0mg/L)+6-BA(0.1mg/L)+IAA(0.1mg/L)和甘氨酸(3.0mg/L)+VB_1(0.6mg/L)+VB_6(0.25mg/L)+烟酸(100mg/L)+肌醇(1.00mg/L),在此组合下,试管苗光照培养30d,形态指标总值和繁殖系数分别为25.52和24.98,达到最佳。The effects of the MS basic mediums adding different concentration of hormone or organics combination on the growth of virus-free sweet potato Ganshu No. 1 seedling rapid propagation were studied in this research. The results indicated that the order of all hormones and organics affecting the sum of the indexes of the virus-free plantlet was as follow： 6-BA 〉 NAA 〉 IAA and niacin 〉 inositol 〉 VB1 〉 glycine 〉 VB6. The optimal combination of hormones and organics were NAA （0mg/L）＋6-BA （0.1mg/L）＋IAA （0. lmg/L） and glycine （3.0mg/L）＋VB1 （0.6mg/L）＋VB6 （0.25mg/ L）＋niacin （100mg/L）＋inositol （1.00mg/L）. The total value of morphological indicators of the seedlings was 25.52, and the index of the rapid propagation was up to 24.98 after culturing under the optimal medium for 30 days.

关 键 词：激素 有机物 甘薯 脱毒苗 快繁 

分 类 号：S531[农业科学—作物学]