EDS1正调控茄子抗青枯病反应  

Bacterial Wilt Resistance of EDS1 Positive Regulating Eggplant

作　　者：李可[1] 肖熙鸥[1] 林文秋[1] 李威[1] 吕玲玲[1] 曹必好[2] LI Ke1, XIAO Xiou1 , LIN Wenqiu1, LI Wei1, LV Lingling1, CAO Bihao2(1 South Subtropical Crop Research Institute of Chinese Academy of Tropical Agricultural Sciences Key Laboratory of tropical fruit biology of Ministry of Agriculture, Zhanjiang, Guangdong, 524091, China 2 College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, Chin)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]农业部热带果树生物学重点实验室、华南农业大学园艺学院,广东广州510642

出　　处：《热带作物学报》2018年第2期332-337,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31501774、No.31672156);海南省自然科学基金(No.20153120);中国热带农业科学院基本科研业务费专项资金(No.1630062017014).

摘　　要：EDS1(Enhanced Disease Susceptibility 1)在植物抗病过程中起着关键的作用。为进一步研究其在调控茄子抗青枯病中的作用,根据茄子基因组序列设计引物克隆了EDS1,将其命名为SmEDS1。生物信息学分析表明SmEDS1最大开放阅读框包含1 809 bp,编码602个氨基酸残基。其包含该基因家族典型的LP结构域和EP结构域。为了进一步验证其功能,通过VIGS技术沉默茄子抗青枯病材料SmEDS1后接种青枯病病原菌,7 d后植株枯萎,结果表明EDS1正调控茄子抗青枯病。半定量结果表明,SmEDS1沉默后对其他信号基因的表达量有着显著影响,其中MAPK6、RAR1的表达量上调,而MAPK3、SIPK、PAD4、SGT1、TGA、EDR1、NPR1、ICS1、GLUA、EIL1和HSP90等基因的表达量下调,EBF2和ACO5的表达量不受SmEDS1的调控。此结果表明EDS1在调控茄子抗青枯病中起着重要的作用。EDS1 （Enhanced Disease Susceptibility 1）plays a key role in plant disease resistance. To further analyze the EDS1 function in the eggplant bacterial wilt resistance, SmEDS1 was cloned according to the eggplant genome sequence. The bioinformation analysis showed that the max ORF box of SmEDS1 was 1 809 bp which coding 602 aa. SmEDS1 contained an LP and EP domain. After the resistant eggplant SmEDS1 gene was silenced by VIGS, the inoculated eggplant showed wilt at 7 days after inoculation with Ralstonia solanacearum. The result suggested that SmEDS1 positively regulated the eggplant bacterial wilt resistance. The gene expression of MAPK6, RAR1 increased while MAPK3, SIPK, PAD4, SGT1, TGA, EDR1, NPR1, ICS1, GLUA, ElL and HSP90 decreased after SmEDS1 was silenced. The expression of EBF2 and ACO5 were not regulated by SmEDS1. The results suggest that EDSI plays an important role in controling eggplant resistance to bacterial wilt.

关 键 词：茄子 SmEDS1 青枯病 

分 类 号：Q945.8[农业科学—植物病理学]