朱砂叶螨为害前后抗、感木薯品种叶组织中无色花青素还原酶活性差异分析  

Analysis of the Change of Activity of Leucoanthocyanidin Reductase in Different Leaf Tissues of Mite-resistant and Mite-susceptible Cassava Cultivars Damaged by Tetranychus cinnabarinus

作　　者：赵惠萍 梁晓[2] 伍春玲[2] 陈青[2] ZHAO Huiping1,2, LIANG Xiao2, WU Chunling2, CHEN Qing2(1 College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China 2 Environment and Plant Protection Institute, China Academy of Tropical Agricultural Sciences/ Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture/ ltainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, Chin)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带作物病虫害生物防治工程技术研究中心海南省热带农业有害生物监测与控制重点实验室,海南海口571101

出　　处：《热带作物学报》2018年第2期349-354,共6页Chinese Journal of Tropical Crops

基　　金：国家木薯产业技术体系虫害防控岗位科学专项(No.CARS-11-HNCQ);海南省科协青年科技人才学术创新计划项目(No.201508);中央级公益性科研院所基本科研业务费专项(No.2017hzs1J013)

摘　　要：为了解木薯单宁酸合成关键酶无色花青素还原酶(leucoanthocyanidin reductase,LAR)在木薯抗螨中的功能与作用,本研究分析朱砂叶螨为害前后抗、感木薯品种不同叶龄组织LAR酶活性变化差异。结果表明:朱砂叶螨为害1、8 d时,高感木薯品种BRA900的未展开叶、展开幼嫩叶、顶芽下第3叶、中下部叶片的LAR酶活性分别为螨害前的1.38倍和1.44倍、1.33倍和1.48倍、1.42倍和1.43倍、0.82倍和0.50倍,除了中下部叶片螨害8 d的LAR酶活性显著降低外,其余叶片LAR酶活性在螨害前后均无显著差异(p<0.05);朱砂叶螨为害1、8 d时,高抗木薯品种C1115的未展开叶、展开幼嫩叶、顶芽下第3叶、中下部叶片的LAR酶活性则分别比螨害前显著提高2.13倍和2.41倍、2.02倍和2.09倍、2.02倍和2.04倍、2.01倍和2.03倍(p<0.05)。上述结果为阐明LAR酶活性显著提高与木薯品种抗螨能力的相关性及田间朱砂叶螨主要为害木薯中下部叶片提供了初步证据。In order to study the function of leucoanthocyanidin reductase（LAR）, a key tannin synthesis enzyme in cassava, the change of activity of LAR in different leaf tissues of mite-resistant and mite--susceptible cassava cuhivars when damaged by T. cinnabarinus was analyzed in this study. The results showed that when the mite-susceptible cassava cultivars BRA900 were damaged for 1 d and 8 d, the activities of LAR in damaged rolled leaves, unfolded young leaves, the third leaves below the bud and the middle and base leaves were 1.38-, 1.44-, 1.33-, 1.48-, 1.42-, 1.43-, 0.82- and 0.50-fold compared to those before damaged, respectively, besides, the activities of LAR in cassava leaves either before and after damaged by T. cinnabarinus did not show significant difference except for those in 8 d-damaged leaves. Moreover, the activities of LAR in 1 d- and 8 d-damaged leaves of mite-resistant cassava cuhivars Cl115 significantly increased to 2.13-, 2.41-, 2.02-, 2.09-, 2.02-, 2.04-, 2.01- and 2.03-fold, respectively. The results provide preliminary evidence for the correlation between the increased activities of LAR and cassava resistance to mite, and partially explained why T. cinnabarinus mainly infested the cassava leaves from middle and base of the nlant.

关 键 词：木薯品种 朱砂叶螨 LAR酶活性 抗螨功能 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]