西门塔尔杂种牛UCP3基因多态性与生长性状的关联性分析  

Polymorphism in UCP3 Gene and Its Association with Growth Traits in Hybrid Simmental Cattle(Bos taurus)

作　　者：蒋秋斐[1] 洪龙 封元 张娟[1] 王瑜 巫亮 顾亚玲[1] JIANG Qiu-Fei1 HONG Long2 FENG Yuan2 ZhANG Juan1WANG Yu2 WU Liang2 Gu Ya-Ling1(1 Agricultural College, Ningxia University, Yinchuan 750021, China; 2 Animal Husbandry Extension Station,Yinehuan 750001, Chin)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏畜牧工作站,银川750001

出　　处：《农业生物技术学报》2018年第4期642-651,共10页Journal of Agricultural Biotechnology

基　　金：宁夏科技厅重大专项(优质高档肉牛新品系选育)(No.2012ZDN0901)

摘　　要：宁夏作为全国重要的肉牛养殖地,肩负着向全国提供优质牛肉的责任。西门塔尔(Bos taurus)杂种牛作为主要养殖群体,更是研究的重点。检测西门塔尔杂种牛解偶联蛋白3(uncoupling protein3,UCP3)基因的遗传多态性,分析其与生长性状的相关性,旨在选择出具有生长速度快的基因类型个体,为下一步选育选配工作提供基础资料。本研究采用PCR-SSCP(polymerase chain reaction-single strand conformation polymorphism)方法结合DNA测序检测UCP3基因的多态性,利用DNAMAM、POPGENE1.31、PIC(Polymorphism information content)程序、SAS软件,计算等位基因频率、基因型频率、多态信息含量,对各个基因型与生长性状(体重、体高、体斜长、胸围)进行关联性分析。UCP3基因共检测到5处点突变,为G5465A、C7921G、T8018C、T8162C、A7953G,其中A7953G突变前后不引起氨基酸改变,其他位点突变后分别引起丙氨酸变成苏氨酸,脯氨酸变成精氨酸,半胱氨酸变成精氨酸,酪氨酸变成组氨酸;对其中两个多态位点进行适合性检验,多态位点Hardy-Weinberg不平衡;遗传多样性分析表明,两个位点的PIC值分别为0.372 7、0.370 4,均表现为中度多态;最小二乘均值分析表明,在第3外显子,GA基因型个体各月龄生长性状优于GG基因型,5 465 bp处发生G→A的突变会影响体重、体高和体斜长,尤其在生长后期,增重优势明显;在第6外显子CC基因型各月龄体尺性状优于TC基因型个体,8 162 bp处发生T→C的突变会影响从初生到18月龄的体高、体斜长的增长速度(P<0.05);将2个突变位点合并进行分析,组合基因型个体(GACC)体重、体高、体斜长均高于其他基因型组合,且体重性状的组合效应值高于单个多态位点。UCP3基因单个或组合位点不同基因型与西门塔尔杂种牛生长性状之间有着显著、极显著的关联,组合GACC基因型不同月龄体重、体斜长优于单个位点。可以将G5465A、T8162C位点作Ningxia which as an important beef cattle breeding province in China has responsibility to provide superior beef meat to whole country. The purpose of that was to study on genetic polymorphism of UCP3 gene in Simmental Hybrid Cattle（Bos taurus） and analyze their association with several growth traits to findgenotype with fast growth rates that provided foundation for selecting and mating. PCR-SSCP and DNA sequencing were used to detect polymorphisms, contained DNAMAM, POPGENE1.31, PIC program, SAS software to calculate allelic frequencies, genotypic frequencies, PIC value, analysis different genotype association with growth traits（body weight, body height, body length, chest girth）. Five SNPs on exons of bovine UCP3 gene were identified： G5465 A, C7921 G, A7953 G, T8018 C, T8162 C, and caused transversion coding from alanine to threonine, proline to arginine, cysteine to arginine, tyrosine to histidine. The result of χ2 showed that two loci were deviated from the Hardy-Weinberg equilibrium. Genetic diversity analysis showed that the PIC values of two loci were 0.372 7, 0.370 4, which represented moderate polymorphism. The result of least square analysis showed that in exon3 fragment, the individuals with GA genotype showed better body sizes values in every months than GG genotype, the mutation of 5 465 bp G→A had significant effects in body weight, height and body length, especially in the later of growth stage, weight gain showed advantages obviously. In exon6 fragment, individuals with CC genotype showed better body sizes values than TC genotype, the mutation of 8 162 bp T→C had significant influence in body height and length from birth to 18 months（P〈0.05）. Furthermore, analysis showed that body measurement traits（BW： body weight; BH： body height; BL： body length） of individuals with combined genotype GACC were higher than those of the other kinds of combinations. The effect of multi-loci combinations on BW were higher than those of single-marker locus. In hybrid simmental cattle

关 键 词：解偶联蛋白3(UCP3) 西门塔尔杂种牛 多态性 生长性状 

分 类 号：S823.9[农业科学—畜牧学]