番鸭攻击行为的转录组学研究  

Transcriptomics Analysis of the Aggressive Behavior of Muscovy Duck(Cairna moschata)

作　　者：王旭平 杨胜林[1] 陆曼 柳诚刚 谭斌 杨汝才 周美迪 王达娜 李潇蒙 WANG Xu-Ping YANG Sheng-Lin LU Man LIU Cheng-Gang TAN Bin YANG Ru-Cai ZHOU Mei-Di WANG Da-Na LI Xiao-Meng(College of Animal Science, Guizhou University/Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550025, Chin)

机构地区：[1]贵州大学动物科学学院/高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025

出　　处：《农业生物技术学报》2018年第4期687-697,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31360566);贵州省教育厅科技成果转化与产业化项目(黔教合KY字[2017]056号);贵州省科技成果应用与产业化计划项目(黔科合成果[2017]-4110号);贵州省科技支撑计划项目(黔科合支持[2017]2539号)

摘　　要：为从分子遗传学角度探讨番鸭(Cairna moschata)出现攻击行为的原因,本实验应用The Medial Recorder 2.0软件对选取的120只生长状况良好的番鸭进行为期1个月(每天24 h)的行为观察记录,并采用瞬时观察法和连续观察法筛选出表现攻击行为的番鸭(实验组)和未出现攻击行为且表现正常的番鸭(对照组)。对筛选出的对照和实验两组番鸭,本实验采用Illumina HiSeq 2000高通量转录组测序技术对实验和对照两组番鸭(每组3次重复)的下丘脑组织的差异表达基因进行筛选,利用基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)数据库对筛选的差异表基因进行功能注释,并进行差异表达基因聚类分析。结果显示,在出现攻击行为番鸭的下丘脑组织中,被注释到GO数据库中的差异表达基因有626个,其中上调基因137个,下调基因489个,参与调控番鸭攻击行为的差异表达基因有26个,其中上调基因4个,下调基因22个,KEGG数据库注释结果显示,这些差异基因显著富集的信号通路为:吞噬体、溶酶体、沙门氏菌感染、MAPK信号通路、孕激素介导的卵母细胞成熟、mTOR信号通路等6个通路。结果还显示在监管行动的细胞骨架和神经活性配体-受体相互作用通路上差异表达基因富集不显著,但富集程度很大,并在该通路上筛选到了番鸭攻击行为差异表达相关基因。通过GO和KEGG数据库的注释,本实验初步筛选出了33个与番鸭攻击行为相关联的候选基因及其调控机制,并发现ERBB信号通路可能是参与番鸭攻击行为调节的关键通路。该实验结果为从分子遗传学角度探讨番鸭攻击行为的发生机制奠定基础。Based on the Molecular genetics to study the causes of the attack behavior of Muscovy ducks（Cairna moschata）, the experiment adopted the software of The Medial Recorder 2.0 to record the behaviors of120 Muscovy ducks in good growth for 1 month（24 h each day）. The use of instantaneous observation and continuous observation screening： Muscovy ducks（experimental group）, which performed aggressive behavior, and Muscovy duck（normal group）, which did not show aggressive behavior. For the selected control and experimental Muscovy ducks, the experiment adopted the Illumina HiSeq 2000 high flux transcriptome sequencing technology to screen the differentially expressed genes in the hypothalamus of Muscovy ducks which were divided into an experimental group and a control group（repeated 3 times in each group）. Making use of the Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG）databases to screened differentially expressed genes. The differentially expressed genes were enriched in gene ontology and KEGG pathway. The results showed that in the hypothalamus of Muscovy ducks which had the attack behavior, there were 626 differentially expressed genes annotated into the GO database, in which 137 genes were up-regulated and 489 genes were down-regulated. There were 26 differentially expressed genes involved in regulating the attack behavior, in which 4 genes were up-regulated and 22 genes were downregulated. The results of KEGG annotation showed that the differentially expressed genes were enriched in six pathways, such as Phagosome, Lysosome, Salmonella infection, MAPK signaling pathway, Progesterone mediated oocyte maturation, m TOR signaling pathway and so on. The results also showed that in the Regulation of action cytoskeleton and the Neuroactive ligand receptor interaction, the enrichment of the differentially expressed genes was not significant, but the enrichment was a very large degree. In these pathways, the differentially expressed genes about the attack behavior of Muscov

关 键 词：转录组 番鸭 下丘脑 差异表达基因 攻击行为 

分 类 号：S834.89[农业科学—畜牧学]