基于网络药理学的二至丸保肝作用机制研究  

Network pharmacology-based study on mechanism of liver protection of Erzhi Pill

作　　者：翟园园 刘其南[1] 徐佳[1] 姚卫峰[1] 包贝华[1] 曹雨诞[1] 张丽[1] 丁安伟[1] ZHAI Yuan-yuan, LIU Qi-nan, XU Jia, YAO Wei-feng, BAO Bei-hua, CAO Yu-dan, ZHANG Li, DING An-wei(Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China)

机构地区：[1]南京中医药大学,江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《药学学报》2018年第4期567-573,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金面上项目资助(81573554);江苏高校"青蓝工程"资助;江苏省"六大人才高峰"高层次人才项目资助(2016-YY026)

摘　　要：构建二至丸保肝的"成分-核心靶点-通路"分子调控网络,探索其"多成分-多靶点-多通路"的作用机制。采用ADME/T计算方法筛选二至丸保肝活性成分,通过网络药理学研究方法,基于反向药效团匹配的靶标识别服务平台分析预测潜在作用靶点,通过生物学信息注释数据库(DAVID)对靶点基因功能及代谢通路进行分析,采用Cytoscape软件构建二至丸保肝"成分-核心靶点-通路"网络模型。结果显示,二至丸中39个主要的保肝活性成分可能通过调控HRAS、DCK、HSD17B1和UCK2等321个靶点,干预胰岛素信号通路、Fox O信号通路、代谢通路和糖酵解过程等51条通路发挥保护肝脏作用,体现了中药多成分、多靶点和多途径的作用特点,为该复方作用机制的阐述提供了新的思路和线索。This study was designed to construct a ＂drug-core target-pathway＂ network of Erzhi Pill for hepatic injury treatment in an effort to explore the ＂multi-components, multi-targets, multi-pathways＂ mechanism. ADME/T calculation method was used to screen the active components of Erzhi Pill, and then predict the potential targets according to the reverse pharmacophore matching method. Biological information annotation databases （DAVID） was used to analyze the molecular function and biological process of the action targets. The Cytoscape software was used to construct the ＂ingredient-core target-pathway＂ network of Erzhi Pill for hepatic injury treatment. It was found that 39 major active ingredients of Erzhi Pill regulated 321 targets （HRAS, DCK, HSD17B1, UCK2, et al） and affected 51 pathways, such as insulin signaling pathway, FoxO signaling pathway, metabolic pathways and glycolysis/gluconeogenesis. The method revealed the action features of traditional Chinese medicine as multi-ingredients, multi-targets, multi-pathways, providing new clues for further basic study on the hepatic injury pharmacological mechanism of Erzhi Pill.

关 键 词：网络药理学 二至丸 女贞子 墨旱莲 保护肝脏 作用机制 

分 类 号：R285[医药卫生—中药学]