补脾强力复方对EAMG大鼠血清CORT、E、ACTH、CRH干预作用的研究  

Interventional effect of compound spleen-tonifying prescription on serum levels of corticosterone,epinephrine,adrenocorticotropic hormone,and corticotropin-releasing hormone in rats with EAMG

作　　者：陈薇[1] 王静 况时祥[3] 何前松[3] 楚兰[4] 张艺凡[4] CHEN Wei , WANG Jing2, KUANG Shi - xiang, HE Qian - song3, CHU Lan4, ZHANG Yi - fan4(1. Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou, China ; 2. Xinzhou Hospital of Traditional Chinese Medicine, Wuhan 430400, Hubei, China ; 3. The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China; 4. Guizhou Medical University, Guiyang 550025, Guizhou, Chin)

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]湖北省武汉市新洲区中医院,湖北武汉430400 [3]贵阳中医学院第二附属医院,贵州贵阳550003 [4]贵州医科大学,贵州贵阳550025

出　　处：《湖南中医杂志》2018年第3期164-166,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81360605)

摘　　要：目的:观察补脾强力复方对实验性自身免疫性重症肌无力(EAMG)大鼠血清皮质醇(CORT)、肾上腺素(E)、促肾上腺皮质激素(ACTH)、促肾上腺皮质激素释放激素(CRH)的影响,探讨补脾强力复方治疗重症肌无力(MG)的可能机制。方法:采用鼠源性AchR-α亚基97-116肽段序列(R97-116)免疫接种雌性Lewis大鼠,建立EAMG大鼠动物模型;通过ELISA法检测EAMG大鼠免疫后第7周CORT、E、ACTH、CRH含量水平,比较补脾强力复方大、中、小剂量与强的松的干预效果。结果:与正常组比较,模型组大鼠血清ACTH、CRH水平显著升高(P<0.05),血清E水平显著降低(P<0.01),CORT水平差异无统计学意义(P>0.05);与模型组比较,补脾强力复方各剂量组及强的松组ACTH、CRH水平显著降低(P<0.01),血清E、CORT水平显著升高(P<0.01);补脾强力复方大剂量组血清ACTH、CRH、E水平更接近正常组水平。结论:补脾强力复方能调节EAMG大鼠血清CRH、ACTH、E、CORT的分泌,能有效降低EAMG大鼠血清ACTH、CRH水平及升高E、CORT水平,其大剂量组使各激素的分泌更接近正常组水平,这可能是补脾强力复方治疗MG的可能机制。Objective： To investigate the interventional effect of compound spleen-tonifying prescription on serum levels of corticosterone（ CORT）,epinephrine（ E）,adrenocorticotropic hormone（ ACTH）,and corticotropin-releasing hormone（ CRH） in rats with experimental autoimmune myasthenia gravis（ EAMG）,as well as the possible mechanism of action of compound spleen-tonifying prescription in the treatment of myasthenia gravis（ MG）. Methods： The mouse-derived AchR-α subunit 97-116 peptide（ R97-116） was used for immunization of female Lewis rats to establish a rat model of EAMG. ELISA was used to measure the levels of CORT,E,ACTH,and CRH at week 7 after immunization,and the interventional effect of high-,middle-,and low-dose compound spleen-tonifying prescription was compared with that of prednisone. Results： Compared with the normal group,the model group had significant increases in the serum levels of ACTH and CRH and a significant reduction in the serum level of E（ P 0. 01）,while there was no significant difference in the level of CORT（ P 0. 05）. Compared with the model group,the high-,middle-,and low-dose compound spleen-tonifying prescription groups and the prednisone group had significant reductions in the levels of ACTH and CRH and significant increases in the serum levels of E and CORT（ P 0. 01）; the high-dose compound spleen-tonifying prescription group had similar serum levels of ACTH,CRH,and E as the normal group. Conclusion： Compound spleen-tonifying prescription can regulate the secretion of CRH,ACTH,E,and CORT in serum,and effectively reduce the serum levels of ACTH and CRH and increase the levels of E and CORT,and high-dose compound spleen-tonifying prescription helps to achieve almost normal secretion of these hormones,which may be one of the mechanisms of action of compound spleen-tonifying prescription in the treatment of MG.

关 键 词：重症肌无力 补脾强力复方 大鼠 实验研究 

分 类 号：R285.5[医药卫生—中药学]