3个玉米WRKY转录因子在非生物胁迫下的表达分析  

Expression Analysis of Three Maize WRKY Transcription Factors under Abiotic Stress

作　　者：决登伟[1] 桑雪莲 舒波[1] 刘丽琴[1] 王一承[1] 石胜友[1] JUE Dengwei,SANG Xuelian, SHU Bo, LIU Liqin, WANG Yicheng and SHI Shengyou(Research Institute of South Subtropical Crops,Chinese Academy of Tropical Agricultural Science Ministry of Agriculture,Zhaniiang Guangdong 524091,Chin)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,农业部热带果树生物学重点实验室,广东湛江524091

出　　处：《西北农业学报》2018年第3期354-361,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：海南省自然科学基金(20163111);国家自然科学基金(31572087).

摘　　要：为进一步研究WRKY转录因子在玉米生长发育及逆境胁迫过程中的作用,通过生物信息方法,从玉米基因组中得到3个同源性较高的WRKY家族基因:ZmWRKY1-like、ZmWRKY4-like、ZmWRKY21-like。同时,采用荧光定量PCR技术分析这3个基因在玉米不同组织及不同逆境胁迫下的表达模式。结果表明,3个基因在玉米的不同器官中都有表达,但具有组织表达特异性。200mol/L NaCl胁迫处理后,ZmWRKY21-like基因呈上调表达;200g/L PEG6000胁迫处理后,ZmWRKY1-like基因出现下调表达;4℃低温胁迫下,3个基因的表达都没有出现显著变化。上述结果表明,ZmWRKY1-like和ZmWRKY21-like基因可能分别参与玉米植株对干旱和盐胁迫的响应。In order to lay a good foundation for research on the function of WRKY family genes in the growth and response mechanisms of stress in maize, we identified three WRKY transcription factor （TF） genes （ ZmWRKYI-like , ZmWRKY4-like , ZmWRKY21-like ） in maize genome by using bioinformatics method. In addition, the expression patterns of three genes in different tissues and under different stresses were analyzed by real-time qPCR. The results showed thatZm WRKYI-like, Zm- WRKY4-like , ZmWRKY21-like expressed in all tested maize tissues and had a differential expres- sion. The expression ofZm WRKY21-like was up-regulated under salt stress and Zm WRKY1-like was down-regulated under drought stress. However, the expression levels of these three genes under low temperature stress had no significant change. These results indicated that ZmWRKYI-like and Zm- WRKY21-Iike may be involved into plant response to drought and salt stress, respectively.

关 键 词：玉米 WRKY转录因子 非生物胁迫 

分 类 号：S513[农业科学—作物学]