三七三醇皂苷对糖尿病大鼠学习记忆功能的改善作用  

Effect and possible mechanism of panaxtrial saponins on cognitive dysfunction in diabetic rats

作　　者：郑涛 左中夫 刘畅 刘学政 ZHENG Tao1, ZUO Zhong-fu1,2, LIU Chang1, LIU Xue-zheng1(1. Department of Human Anatomy, Jinzhou Medical University, Jinzhou 121001; 2. Department of Human Anatomy, Guangxi Medical University, Nanning 550021, Chin)

机构地区：[1]锦州医科大学解剖教研室,辽宁锦州121001 [2]广西医科大学解剖教研室,广西南宁530021

出　　处：《解剖科学进展》2018年第2期188-190,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81571383);中国博士后科学基金(2017M612870);辽宁省高等学校杰出青年学者成长计划(LJQ2015069);辽宁省自然科学基金联合基金(201602340);锦州医科大学大学生科技创新基金(201610160014)

摘　　要：目的探讨三七三醇皂苷(panax notoginseng,PTS)对糖尿病大鼠学习记忆的改善作用及可能机制。方法链脲佐菌素诱导SD大鼠糖尿病模型,将大鼠分为对照组、糖尿病组及PTS组,3个月后水迷宫实验检测大鼠学习记忆,Nissl染色观察脑海马区神经元形态,TUNEL染色观察海马区神经元凋亡,免疫组化染色和Western blot检测凋亡相关基因Bax在海马区的表达情况。结果与对照组相比,糖尿病组大鼠学习记忆能力减退,表现为逃避潜伏期长以及穿经水迷宫平台次数减少,而且脑海马区神经元形态不规则,排列紊乱,结构不清晰,凋亡神经元及Bax表达均明显增加。PTS组与对照组无明显差别。结论PTS改善糖尿病大鼠学习记忆能力可能与下调糖尿病脑海马区神经元Bax的表达,抑制神经元凋亡相关。Objective To explore the beneficial effect of panaxtrial saponins （PTS） on cognitive dysfunction of the diabetic SD rats. Methods SD rats were intraperitoneally injected with streptozotocin to induce diabetic models. Rats were divided into PTS, diabetic model and control groups. Learning and memory ability was observed by Water maze, TUNEL and Nissl staining were used to reveal the morphological and apoptosis alterations in hippocampal neurons, and the expression of apoptosis-related Bax in hippocampal was observed by immunohistochemistry and Western blot. Results Compared with control group, diabetic group showed cognitive dysfunction in water maze test, presenting increased escape latency and decreased cross number, together with increased apoptosis, disarranged neurons showing unclear structure, and up-regulation of Bax expression, while, PTS group showed no difference compared with control group. Conclusion Panaxtrial saponins ameliorating learning and memory ability might be related to the inhibition of Bax expression and apoptosis in hippocampal neurons of diabetic rats.

关 键 词：三七三醇皂苷 糖尿病 学习记忆 BAX 凋亡 SD大鼠 

分 类 号：R587.1[医药卫生—内分泌]