中国六省份水痘-带状疱疹病毒糖蛋白M、L基因特征分析  

Characterization analysis of gM, gL genes of varicella zoster virus in six provinces of China

作　　者：牟君杰 齐梦缘 李崇山[2] 郑焕英[3] 陈萌[4] 周剑慧[5] 何吉兰[6] 许文波[7] 许松涛[7] 许晓光[1] Mou Junjie,Qi Mengyuan, Li Chongshan, Zheng Huanying, Chen Meng, Zhou Jianhui, He Jilan, Xu Wenbo, Xu Songtao, Xu Xiaoguang(Institute of Integrative Medicine of Dalian Medical University, Dalian 116044, Chin)

机构地区：[1]大连医科大学中西医结合研究院,116044 [2]上海市疾病预防控制中心卫生检验所 [3]广东省疾病预防控制中心卫生检验所 [4]北京市疾病预防控制中心免疫所 [5]吉林省疾病预防控制中心免疫所 [6]四川省疾病预防控制中心微检所 [7]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华预防医学杂志》2018年第4期419-423,共5页Chinese Journal of Preventive Medicine

基　　金：国家重点研发计划（2016YFC1200905）;国家自然科学基金（81302477）

摘　　要：目的 分析水痘-带状疱疹病毒（VZV）糖蛋白（g）M、L的基因特征。方法 依据国家科技重大专项中发热伴出疹症候群病毒性病原监测项目,以2007—2015年北京（1例）、上海（5例）、吉林（2例）、青海（1例）、广东（2例）、四川（1例）监测到的12例VZV疑似患者为研究对象，收集其疱疹液或咽拭子标本共12份。通过琼脂糖凝胶电泳鉴定标本VZV阳性情况，采用PCR扩增VZV阳性标本以及国产水痘减毒活疫苗v-Oka-BK和进口水痘减毒活疫苗Varilrix-1的gM、gL基因序列片段，进行核苷酸序列测定和分析。采用BioEdit、MEGA 5.0软件，比对VZV阳性标本与疫苗株（v-Oka-BK、varilrix-1）以及GenBank的国外野毒株（41株）、亲本株（p-Oka）、疫苗株（v-Oka、varilrix、varivax）的核苷酸序列。结果 12份标本均为VZV阳性。与疫苗株和亲本株相比，1份阳性标本gM基因在86686位点发生碱基突变，并导致氨基酸突变，5份阳性标本在87844位点发生碱基突变，有30株国外野毒株在87844位点上也存在相同变异；1份阳性标本gL基因在101245位点发生碱基突变，并导致氨基酸突变，6份阳性标本在101624、101625、101625位点上均发生了碱基缺失，国外野毒株在这3个位点均发生相同变异。与疫苗株相比，12份VZV阳性标本gM的核苷酸、氨基酸同源性分别为99.2%~100%和98.2%~100%，gL的分别为99.3%~100%和98.6%~100%；与41株国外野毒株相比，gM的核苷酸、氨基酸同源性分别为99.3%~100%和98.5%~100%，gL的分别为99.1%~100%和98.6%~100%。基因亲缘性关系树分析显示，基于gM有7份VZV阳性标本与疫苗株及亲本株在同一分支上；基于gL，有12份VZV阳性标本均与疫苗株及亲本株在同一分支。结论 12份VZV阳性标本gM、gL基因序列高度保守，抗原性稳定，存在减毒位点可能。Objective To analyze the genetic characterization of glycoprotein M（gM.）,glycoprotein L（gL） of varicella zoster virus.Methods According to the program of ＂Ministry of Science and Technology of China＂ , Based on the 12 suspected VZV patients monitored in Beijing （1 case）, Shanghai （5 cases）, Jilin （2 cases）, Qinghai （1 case）, Guangdong （2 case） and Sichuan （case） in 2007-2015. A total of 12 Vesicle fluid and throat swab samples were collected. Positive samples were identified by Agarose gel electrophoresis and two glycoprotein genes were amplified by polymerase chain reaction （PCR）. Nucleotide sequences were determined and analyzed by PCR amplification of VZV positive specimens V-OKA-BK of the domestic varicella attenuated live vaccine and the Varilrix-1 of the imported attenuated live vaccine. Nucleotide sequences of VZV positive specimens, vaccine strains （V-OKA-BK, varilrix-1） and GenBank foreign wild strains （41 strains）, parent strains （P-oka）, vaccine strains （V-oka, Varilrix, Varivax） were compared using BioEdit and MEGA 5.0.Results 12 specimens were VZV positive. Compared with the vaccine strains and the parent strains, the GM gene of 1 positive specimen had radical mutation at 86686 sites, which resulted in amino acid mutation, 5 positive specimens had base mutation at 87844 sites, and 30 strains of foreign wild strains had the same variation at 87 844 sites. 1 positive specimens of gL gene in 101245 sites had base mutation, and led to amino acid mutation, 6 positive specimens at 101624, 101625, 101626 sites had base of loss and the foreign wild strains in these 3 sites had the same variation. Compared with the vaccine strains, the nucleotide and amino acid homology of gM of 12 VZV positive specimens were 99.2%-100% and 98.2%-100%, respectively, and gL of those were 99.3%-100% and 98.6%-100%, respectively. Compared with 41 strains of foreign wild strains, homology of gM＇s nucleotides and amino acid were 99.3%-100% and 98.5%-100%, respectively; 9

关 键 词：糖蛋白类 水痘疫苗 基因特征 水痘.带状疱疹病毒 

分 类 号：R440[医药卫生—诊断学]