异补骨脂素对紫外线诱导人真皮成纤维细胞损伤的保护作用及机制研究  

Protctive effects of Angelicin on UVB-induced HDF cells damage and its mechanism

作　　者：刘俊岑 刘海洋[2] 刘国良[2] 王月莹 高海南 耿放[3] 张宁[2] Liu Juneen, Liu Haiyang, Liu Guoliang, Wang Yueying, Gao Hainan, Geng Fang, Zhang Ning(Master Grade of 2015, Heilongjiang University of Chinese Medicine, Harbin 150040, Chin)

机构地区：[1]黑龙江中医药大学研究生学院,哈尔滨150040 [2]黑龙江中医药大学佳木斯学院,154007 [3]哈尔滨师范大学化学化工学院,150025

出　　处：《国际中医中药杂志》2018年第4期339-344,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（81274035）;黑龙江省博士后科研启动金（LBH-Q13162）;黑龙江中医药大学优秀创新人才支持计划（2012RCD22）

摘　　要：目的 研究异补骨脂素对人真皮成纤维细胞（human dermal fibroblasts, HDF）增殖活性及抗老化相关蛋白表达的影响，并探讨其作用机制。方法 将细胞按随机数字表法分为空白组、模型组、雌二醇组、异补骨脂素组、雌激素受体拮抗剂＋雌二醇组、雌激素受体拮抗剂＋异补骨脂素组、P38通路阻断剂组。分别给予不同药物进行干预24 h。除空白组外，其余各组细胞给予紫外线照射，照射剂量为150 mJ/cm2。采用MTT法检测细胞增殖率，采用Western blot法检测细胞中胶原蛋白Ⅰ（collagenⅠ, COLⅠ）、MMP-1、雌激素受体b（estrogen receptor b, ERb）、P38、p-P38蛋白表达量，实时荧光定量PCR法检测MMP-1 mRNA表达。结果 与模型组比较，异补骨脂素10、1、0.1、0.01 μmol/L组HDF细胞增殖率升高（P＜0.01）；异补骨脂素10 μmol/L组HDF细胞COLⅠ[（0.326±0.006）比（0.176±0.007）]、ERb[（0.281±0.011）比（0.143±0.006）]蛋白表达升高（P＜0.01），MMP-1[（0.256±0.006）比（0.395±0.006）]和p-P38[（0.224±0.003）比（0.318±0.005）]蛋白表达降低（P＜0.01）；与异补骨脂素10 μmol/L组比较，雌激素受体拮抗剂＋异补骨脂素组ERb[（0.120±0.007）比（0.281±0.011）]蛋白表达下降、MMP-1 mRNA[（1.377±0.012）比（1.024±0.010）]表达升高（P＜0.01）。结论 异补骨脂素可能通过ER-P38 MAPK信号通路使MMP-1降解，进而促进胶原合成，达到预防和治疗光老化的目的。Objective To study the effect of Angelicin on proliferation activity and anti-aging related protein expression of human HDF cells and its mechanism. Methods According to the random number table method, the cells were divided into blank group, model group, estradiol group, Angelicin group, estrogen receptor antagonist＋estradiol group, estrogen receptor antagonist＋Angelicin group, and P38 pathway blocker group. Different groups were given the according drugs respectively for 24 h. Except the blank group, all the groups of cells were given UVB irradiation with a dose of 150 mJ/cm2. The MTT assay was used to detect cell proliferation rate. The Western blot was used to detect the expression levels of COLⅠ, MMP-1, ERβ, P38 and p-P38 in cells, and the MMP-1 mRNA expression was detected by real-time fluorescent quantitative PCR. Results Compared with the model group, the proliferation rate of HDF cells significantly increased in Angelicin （10, 1, 0.1 and 0.01 μmol/L groups） （P〈0.01）; The protein expression of COLⅠ （0.326 ± 0.006 vs. 0.176 ± 0.007）, ERb （0.281 ± 0.011 vs. 0.143 ± 0.006） significantly increased （P〈0.01）, and the expression of MMP-1 （0.256 ± 0.006 vs. 0.395 ± 0.006） and p-P38 （0.224 ± 0.003 vs. 0.318 ± 0.005） significantly decreased （P〈0.01） in Angelicin 10 μmol/L group. Compared with 10 μmol/L Angelicin group, the protein expression of estrogen receptor antagonist＋Angelicin group ERβ （0.120 ± 0.007 vs. 0.281 ± 0.011） significantly decreased and MMP-1mRNA （1.377 ± 0.012 vs. 1.024 ± 0.010） significantly increased （P〈0.01）. Conclusions The Angelicin may degrade MMP-1 through the ER-P38 MAPK signaling pathway,and then promote collagen synthesis, to achieve the purpose of prevention and treatment of photoaging.

关 键 词：白芷素 雌激素受体beta p38有丝分裂原激活蛋白激酶类 胶原 基质金属蛋白酶类 皮肤衰老 

分 类 号：R285.5[医药卫生—中药学]