聚丙烯酸钠-海藻酸钠-聚天冬氨酸凝胶球脱除鱿鱼内脏酶解液中的铅  

Removal of Lead from Enzymatic Hydrolysate of Squid Viscera Protein by Sodium Polyacrylate-Sodium Alginate-Polyaspartic Acid Gel Beads

作　　者：陈宏[1,2,3] 何蒙 张晗[1,2] 张羽 刘旭[1,2] 沈琳 马永钧[5] 劳敏军 励建荣[2,3] Chen Hong1,2,3, He Meng1,2, Zhang Han1,2, Zhang Yu1,2, Liu Xu1,2 ,Shen Lin4, Ma Yongjun5, Lao Minjun5, Li Jianrong2,3(1College of Chemistry and Chemical Engineering, Bohai University, Jinzhou 121013, Liaoning; 2College of Food Science and Technology, Bohai University, Jinzhou 121013, Liaoning ;3National ＆ Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, Liaoning;4Dalian Donglin Food Co., Ltd, Dalian 116001, Liaoning ;5Zhejiang Xingye Group Co., Ltd, Zhoushan 315200, Zhejian)

机构地区：[1]渤海大学化学化工学院,辽宁锦州121013 [2]渤海大学食品科学与工程学院,辽宁锦州121013 [3]生鲜农产品贮藏加工及质量安全控制技术国家地方联合工程技术研究中心,辽宁锦州121013 [4]大连东霖食品股份有限公司,辽宁大连116001 [5]浙江兴业集团有限公司,浙江舟山315200

出　　处：《中国食品学报》2018年第3期81-89,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金："十二五"国家科技支撑计划(2012BAD29B06);辽宁省食品安全重点实验室暨辽宁省高校重大科技平台开放课题(LNSAKF2011038);辽宁省科学事业公益研究基金项目(2012003001)

摘　　要：用聚丙烯酸钠-海藻酸钠-聚天冬氨酸(PAAS-SA-PASP)凝胶球作为吸附剂脱除鱿鱼内脏酶解液中的铅。通过单因素试验研究了凝胶球组成、脱除时间、p H、盐度对铅脱除率的影响。使用响应面分析法获得铅的最佳脱除条件:p H值2.25,脱除时间25.60 h,盐度(氯化钠浓度)0.02 mol/L。在此条件下铅的理论脱除率达到90.05%。测得鱿鱼内脏酶解液试样中铅的脱除率为87.64%~89.10%,脱除后酶解液中的铅含量均达到国家标准。铅脱除导致酶解液蛋白质的损失低于10%,PAAS-SA-PASP凝胶球可多次重复使用,重复使用5次后铅的脱除率仍可达85.37%。A novel hybrid gel bead composed of sodium polyacrylate（PAAS） and alginate（AS） and sodium poly（aspartic acid）（PASP） was prepared, characterized and applied for the removal of lead from the enzymatic hydrolysate of squid viscera protein. Effects of gel composition, removal time, p H and salinity on the removal of lead were studied.Optimization of conditions for lead removal was investigated using response surface methodology（RSM） with three independent variables, removal time, p H, and salinity. The maximum percent removal achieved was 90.05% under the optimal conditions of 2.25 for p H, 25.6 h for removal time, and 0.02 mol/L Na Cl for salinity. The experimental results showed that the percents of lead removal in enzymatic hydrolysates of squid viscera protein were 87.64%-89.10% with the relative standard deviations of 2.57%-3.92%. The loss of squid viscera protein caused by lead removal was below10%. PAAS-SA-PASP can be reused for many times and the percent removal of lead could still be maintained at85.37% level at the fifth cycle.

关 键 词：凝胶球 鱿鱼内脏蛋白 酶解液 铅 脱除 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]