妊娠对肺动脉高压肺循环影响的实验研究  

The effect of pregnancy on pulmonary circulation in rats complicated with pulmonary arterial hypertension

作　　者：李月 金沐[1] 景赫[1] 孙雨薇 程卫平[1] LI Yue;JIN Mu;JING He;SUN Yuwei;CHENG Weiping(Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University,Beijing Institution of Heart, Lung and Blood Vessel Diseases, Beijing 100029, Chin)

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所麻醉科,100029

出　　处：《心肺血管病杂志》2018年第3期253-258,共6页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：目的:复制妊娠合并肺动脉高压大鼠模型,观察妊娠对肺循环的影响,为妊娠肺动脉高压发病机制和治疗研究奠定基础。方法:随机将71只,雌性,6周龄,SD大鼠分为四组:空白对照组(n=15),野百合碱组(MCT,n=20),妊娠组(n=16),妊娠MCT组(n=20)。饲养1周后,给MCT组及妊娠MCT组大鼠颈背部皮下注射60mg/kg MCT诱导形成肺动脉高压。2周后,妊娠组及妊娠MCT组大鼠进行合笼饲养,观察到阴栓的雌鼠被认为合笼成功。去除死亡及合笼失败的大鼠,各组最终分组如下,空白对照组(n=15),MCT组(n=12),妊娠组(n=12),妊娠MCT组(n=12)。合笼成功后于第18天利用股动脉及肺动脉插管的方法进行血流动力学数据收集,同时进行肺组织标本采集。肺组织经切片染色后显微镜下观察肺小动脉损伤情况。结果:MCT组及妊娠MCT组各自的PAP(肺动脉压)、PAP/SAP(体循环动脉压)、肺小动脉相对中膜厚度、肺小动脉密度及增厚小动脉比例均比妊娠组及空白对照组显著升高(P<0.05)。妊娠MCT组大鼠PAP及PAP/SAP明显低于MCT组(P<0.05),但肺小动脉密度增长更显著(P<0.01)。同时妊娠组比空白对照组有更厚的肺小动脉相对中膜厚度(P<0.01)。结论:本研究成功复制了妊娠合并肺动脉高压大鼠模型,发现妊娠在缓解肺高压大鼠肺循环压力增高的同时,对肺小血管的基本形态有显著影响,使肺血管阻力增高。Objective： We planned to creat a pregnant PAH（ pulmonary arterial hypertension） rat model and observe the effect of pregnancy on pulmonary circulation in this model. Methods： The female rats were randomized into 4 groups： control group（ n = 15）,monocrotaline（ MCT） group（ n = 20）,pregnant group（ n = 16） and pregnant MCT（ n = 20） group. A single dose of MCT（ 60 mg/kg,sc） was administered in the back of the pregnant MCT and MCT rat＇s neck to induce PAH at the age of 7 weeks old. The rats of pregnancy and pregnant MCT groups were placed with males for mating 2 weeks later. Vaginal plug was the criterion to judge the success of mating. Eighteen days later,hemodynamic data were recorded by femoral and pulmonary artery intubation. Pulmonary tissues were collected for pulmonary arterial morphometry at the same time. Results： Except for the dead and nonpregnant ones,the final number of four groups were： 15 in control group,12 in MCT group,12 in pregnancy group and 12 in pregnant MCT group. Compared to animals of pregnancy and control groups,the rats of other two groups had greater pulmonary arterial pressure（ PAP）（ P〈0. 01） and the ratio of PAP/SAP（ systemic arterial pressure）（ P〈0. 05）. From the pulmonary vascular specimens of rats in MCT and pregnant MCT groups,we observed increased relative medial thickness,higher occluded arterial density（ P〈0. 01） and lower non-thickened arteries density（ P〈0. 01） compared to rats in the other two groups.Thus,the model was established. It also showed decreased PAP（ P〈0. 01） and ratio of PAP/SAP（ P〈0. 05）,but increased thicked arterial density in pregnant MCT rats than MCT rats（ P〈0. 01）. The pregnant rats showed increased relative medial thickness than controls（ P〈0. 01）. These findings proved that pregnancy is a influence factor to the pulmonary circulation of rats. Conclusion： The present study esbablish a novel model of PAH during pregnancy in rat. The bidirectional effects of pregnan

关 键 词：肺动脉高压 妊娠 模型建立 大鼠 

分 类 号：R54[医药卫生—心血管疾病]