白术中3种内酯成分一测多评方法的建立  

Quantitative Analysis of Multi-Components by Single Marker for Determination of Three Atractylenolides in Atractylodis Macrocephalae Rhizoma

作　　者：赵玉娇[1] 梁国锐 宫文霞[1] 田俊生[1] 秦雪梅[1,2] ZHAO Yu-jiao, LIANG Guo-rui2, GONG Wen-xia1 , TIAN Jun-sheng1 , QIN Xue-mei1＇2.(1. Modern Research Cen- ter for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; 2. Taiynan Tiartzhong Yiyao Technology Co. Ltd. , Taiyuan 030006, Chin)

机构地区：[1]山西大学中医药现代研究中心,太原030006 [2]太原天中益耀科技有限公司,太原030006

出　　处：《中国药学杂志》2018年第7期499-503,共5页Chinese Pharmaceutical Journal

基　　金：国家国际科技合作计划项目资助(2011DFA32630);山西省科技计划项目资助(201603D321077;201603D3113013);山西省科技创新重点团队项目资助(201605D131045-18);山西省重点实验室项目资助(201605D111004)

摘　　要：目的建立白术中3种内酯成分的一测多评方法。方法采用超高效液相色谱法,以乙腈-水为流动相进行梯度洗脱,检测波长为235 nm,进样量为2μL,流速为0.2 mL·min^(-1),柱温30℃。以白术内酯Ⅲ为内参物,测定其与白术内酯Ⅰ、Ⅱ的相对校正因子,利用该相对校正因子计算其他2种成分的含量,同时利用外标法测定3种内酯的含量,比较2种结果的差异,验证一测多评法的可行性和准确性。结果在线性范围内,白术内酯Ⅲ与白术内酯Ⅰ、Ⅱ的相对校正因子分别为0.633、1.895,在不同实验条件下重现性良好,3种成分含量计算值与实测值间无显著性差异。结论所建立的一测多评法可用于白术中3种内酯成分的含量测定,可为白术的质量控制提供科学的理论依据。OBJECTIVE To establish a quantitative analysis of multi-components by single marker（QAMS） for the determination of three atractylenolides in Atractylodes macrocephala Koidz. METHODS UPLC method was applied to determine atractylenolide I , Ⅱ and Ⅲ by external standard method （ESM）first. With this established UPLC method, atractylenolide Ⅲ was used as the inter- nal standard （IS）to determine the correction factors （RCFs）of the two other atractylenolides, and their contents in all the samples were calculated by their RCFs at the same time. By comparing the contents determined by the ESM and QAMS methods, the feasibility and accuracy of QAMS method were verified. RESULTS Within a certain range （ atractylenolide I ： 3.7 - 59.3 mL·min-1, atracty- lenolide Ⅱ ： 3.9 -63.5 mL·min-1 , atractylenolide Ⅲ ： 7.3 - 116.1 mL·min-1 ） , the RCFs of atractylenolide I , Ⅱ to at- ractylenolide Ill were 0. 633 and 1. 895, respectively, which had good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method（P 〉 0.05）. CONCLUSION The QAMS method is feasible and accurate for the determination of the three atractylenolides and is beneficial to the quality control of Atractylodes mac- rocephala Koidz..

关 键 词：白术 相对校正因子 一测多评 白术内酯 质量控制 

分 类 号：R284[医药卫生—中药学]