快速溶剂萃取高效液相色谱法测定蔬菜中烯禾啶和麦草畏残留  

HPLC Determination of Sethoxydim and Dicamba in Vegetables with Accelerated Solvent Extraction

作　　者：金党琴[1] 丁邦东[1] 龚爱琴[1] 王元有[1] 徐洁[1] 周慧[1] JIN Dang-qin;DING Bang-dong;GONG Ai-qin;WANG Yuan-you;XU Jie;ZHOU Hui(Chemical Engineering Institute, Yangzhou Polytechnic In- stitute, Yangzhou 225127, China)

机构地区：[1]扬州工业职业技术学院化学工程学院

出　　处：《化学试剂》2018年第4期357-360,共4页Chemical Reagents

基　　金：扬州市重点研发计划项目(YZ2015030);江苏省青蓝工程项目(苏教师[2014]23号、苏教师[2016]15号);扬州工业职业技术学院校级重点课题项目(2015XK01);江苏省高校自然科学基金资助项目(16KJB150044);江苏省高等学校优秀科技创新团队资助项目(苏教科[2017]6号);江苏省第五期“333高层次人才培养工程”资助项目(苏人才[2016]7号,BRA2017479)

摘　　要：用乙腈作为提取剂快速萃取蔬菜中的烯禾啶和麦草畏,采用高效液相色谱法进行烯禾啶和麦草畏残留量测定。使用Dionex C_（18）色谱柱,以V（乙腈）∶V（3%甲酸）=80∶20为流动相、流速为1.0 m L/min、紫外检测器、检测波长为252 nm,建立一种从蔬菜中快速溶剂萃取烯禾啶和麦草畏结合高效液相色谱法测定其残留量的方法。结果表明,烯禾啶和麦草畏保留时间为4.87、3.84 min,线性相关系数为0.997 8和0.998 2。在7个质量分数添加水平范围内,回收率分别为93.24%~102.51%和91.00%~104.14%,相对标准偏差（RSD）分别为1.93%~5.92%和3.51%~6.11%,方法检出限分别为0.012、0.020 mg/kg。该方法简单、快速、灵敏度高、准确度好,能够满足蔬菜中烯禾啶和麦草畏残留量的检测要求。Sethoxydim and dicamba in vegetables were accelerated extraction of acetonitrile,and were determined by HPLC.Samples were separated on the Dionex C_（18）column with a mobile phase of acetonitrile-3%formic acid（80∶20,by volum）at the speed of 1.0 m L/min.The UV detector wavelength was 252 nm.A method was described for determination of sethoxydim and dicamba residues by high-performance liquid chromatography（HPLC）with accelerated solvent extraction in vegetables.The retention times for sethoxydim and dicamba were 4.87 and 3.84 min,the linear correlation coefficients were 0.997 8 and 0.998 2,respectively.At the seven spiked levels,the recoveries for sethoxydim and dicamba were 93.24%~102.51%and 91.00%~104.14%,the relative standard deviations were 1.93%~5.92%and 3.51%~6.11%,the limits of detection were 0.012 and 0.020 mg/kg,respectively.The method is simple,rapid,sensitive and accurate,which can meet the detection requirements for sethoxydim and dicamba residues in vegetables.

关 键 词：快速溶剂萃取 高效液相色谱 烯禾啶 麦草畏 蔬菜 

分 类 号：O657.7[理学—分析化学]