唾液酸化人乳寡糖的分离及串联质谱结构序列分析  

Separation and Structural Sequence Analysis of Sialylated HMOs via Tandem Mass Spectrometry

作　　者：郎银芝 刘世龙 王晨 张晓[1] 吕友晶[1] 蔡超[1] 李国云[1] 于广利[1] LANG Yinzhi;LIU Shilong;WANG Chen;ZHANG Xiao;LU Youjing;CAI Chao;LI Guoyun;YU Guangli(Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, Chin)

机构地区：[1]山东省糖科学与糖工程重点实验室,海洋药物教育部重点实验室,中国海洋大学医药学院,青岛266003

出　　处：《高等学校化学学报》2018年第4期645-652,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号：31670811); 国家自然科学基金山东海洋科学研究中心联合基金(批准号：U1406403); 山东省科技项目(批准号：2015ZDJS04002,LKH2015102); 泰山学者工程专项基金资助

摘　　要：基于不同唾液酸化寡糖之间的极性、空间结构及电荷密度的差异性,采用半制备型多孔性石墨化碳(PGC)色谱分离制备了高纯度唾液酸化人乳寡糖单体,并使用离线的电喷雾-串联质谱技术(ESI-MSn)确定8种寡糖单体的序列结构分别为6'-SL,3'-SL,LSTb,LSTc,F-LSTc,F-LSTb,DSLNT与FS-LNn H.本文结果为进一步从事唾液酸化人乳寡糖的结构与活性研究提供了参考依据.Sialylated human milk oligosaccharides（ SHMOs）, important components of human milk oligosaccharides,play a key role in the growth and development of the newborn. Herein,based on the difference of polarity,spatial structure and charge density of sialylated oligosaccharides,a semi-preparative porous graphitized carbon（ PGC） chromatography was employed to separate SHMOs in high purity. An off-line electrospray ionization tandem mass spectrometry（ ESI-MSn） was performed to identify the sequential structures of eight oligosaccharides including 6＇-SL,3＇-SL,LSTb,LSTc,F-LSTc,F-LSTb,DSLNT and FS-LNn H. This study provides a useful reference for the further structure-activity analysis and application development of SHMOs.

关 键 词：唾液酸化人乳寡糖 石墨化碳色谱分离 串联质谱 结构序列 

分 类 号：O657[理学—分析化学] O629.1[理学—化学]