分子印迹纳米管膜的制备及对人体尿液中儿茶酚胺类药物的检测  

Preparation of a Molecularly Imprinted Polymer Nanotubes Membrane and Its Application in the Determination of Catecholamines in Urine Samples

作　　者：丘秀珍[1] 华永标 郭会时[1] 卢文贯[1] QIU Xiuzhen;HUA Yongbiao;GUO Huishi;LU Wenguan(College of Chemistry and Environmental Engineering, Shaoguan University, Shaoguan 512005, China)

机构地区：[1]韶关学院化学与环境工程学院,韶关512005

出　　处：《高等学校化学学报》2018年第4期653-659,共7页Chemical Journal of Chinese Universities

基　　金：广东省自然科学基金(批准号:2014A030307024;2016A030307048)资助~~

摘　　要：以多巴胺(DA)为模板,多孔阳极氧化铝膜(AAO)为反应载体,合成了多巴胺分子印迹聚合物纳米管膜(AAO@MIP).利用扫描电子显微镜对分子印迹纳米管膜的形貌进行了表征,并用高效液相色谱(HPLC)研究了其对儿茶酚胺类(CLs)药物的吸附性能.实验结果表明,在最优萃取条件下,AAO@MIP纳米管膜对多巴胺、肾上腺素和去甲肾上腺素具有较高的选择性,3种儿茶酚胺类药物在0.50~300μmol/L浓度范围内呈良好的线性关系(r^2>0.9970);检出限(S/N=3)分别为15.5,12.6和22.5 ng/L.AAO@MIP纳米管膜对多巴胺的最大吸附容量可达82.1μmol/g;6次吸附-解吸附重复利用后,吸附容量仅降低3.3%.将AAO@MIP纳米管膜应用于萃取人体尿液中3种儿茶酚胺,样品加标回收率为74.0%~100.4%,相对标准偏差(RSD)为3.6%~6.8%.该方法简便、快速、选择性高,适用于检测人体尿液中的儿茶酚胺类药物的含量.A molecularly imprinted nanotubes membrane（ AAO@ MIP） was prepared using dopamine（ DA）as template,porous anodic alumina（ AAO） as reactor. The morphology of AAO @ MIP was characterized by means of scanning electron microscopy（ SEM）. The specific adsorption performance of AAO @ MIP to catecholamines（ CLs） was investigated by high performance liquid chromatography（ HPLC）. Results of adsorption experiments revealed that the AAO@ MIP has good extraction capacity and selectivity to dopamine,epinephrine and norepinephrine. A good linearity was achieved for CLs over the range of 0. 50—300 μmol/L and the limit of detection（ S/N = 3） was found to be as low as 15. 5,12. 6 and 22. 5 ng/L for dopamine,epinephrine and norepinephrine,respectively. The maximum adsorption capacity of AAO@ MIP to dopamine can reach 82. 1 μmol/g. And the adsorption capacity is only reduced by 3. 3% after six times adsorptiondesorption. The proposed method was applied to the determination of CLs in human urine samples. The average recoveries ranged from 74. 0% to 100. 4% with the relative standard deviation（ RSD） of 3. 6%—6. 8%. The developed method is rapid,selective and sensitive,and could be adapted to the analysis of CLs in urine samples.

关 键 词：多孔阳极氧化铝膜 分子印迹纳米管膜 儿茶酚胺 高效液相色谱 

分 类 号：O657[理学—分析化学]