黄藤素与临床常用抗菌药物体外联合抗菌作用研究  

In vitro synergistic interactions between palmatine and commonly used antibiotics

作　　者：李耘[1] 吕媛[1] 刘健[1] 薛峰[1] 杨维维[1] 张佳[1] 单爱莲[1] LI Yun, LU Yuan, LIU Jian, XUE Feng, YANG Wei - wei, ZHANG Jia, SHAN Ai - lian(Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100034, Chin)

机构地区：[1]北京大学第一医院临床药理研究所,北京100034

出　　处：《中国临床药理学杂志》2018年第7期821-823,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的评价黄藤素与临床常用抗菌药物对我国近3年临床分离主要致病菌的体外联合抗菌作用。方法对118株临床分离菌及2株标准菌株进行联合药敏和最低抑菌浓度(MIC)测定。联合药敏测定采用棋盘法,MIC测定采用琼脂二倍稀释法。结果黄藤素单药对葡萄球菌有一定抗菌作用,与左氧氟沙星联合,对革兰氏阳性菌、阴性菌均可出现协同作用,协同比例≤20%。与利奈唑胺基本无协同作用。但黄藤素与两药联合具有较高的相加作用比例。结论体外联合抗菌活性研究在不同菌种、不同研究中存在一定差异,可能与研究方法不同、菌株耐药背景不同有关。因此,未来的研究一方面需要更深入开展中药作用机制研究,另一方面引入临床数据,考察体外与临床相关性,将对临床合并用药的选择更有指导意义。Objective To evaluate in vitro synergy between palmatine and commonly used antibiotic against main clinical isolated strains in re- cent three years. Methods All of 118 strains isolated in recent three years and 2 standard strains were studied. Minimal inhibition concentra- tions （MIC） were tested by agar dilution method and combination effects were measured by checkerboard method. Results Palmatine showed moderate in vitro activity against Staphylococcus. Synergy was detected with palmatine in combination with levofloxacin against gram - positive and negative strains,though synergistic rate less than 20%. Synergy was not show between palmatine and linezolid. Additive effect was found widely between palmatine and two agents against almost all tested spe- cies. Conclusion The results of in vitro synergy tests may be different sometimes on account of various strains, methods, etc. So, more deeper understanding of action mechanism of traditional Chinese medicine and correlation between in vitro test and clinical outcomes will help medical to select antibiotic more suitablly.

关 键 词：黄藤素 利奈唑胺 左氧氟沙星 体外 联合药敏 

分 类 号：R978.1[医药卫生—药品]