芍药总苷对6MV-X射线放射性食管炎模型大鼠的作用研究  

Effect of total glucosides of peony on esophagitis model of rats by 6MV-X ray radiation

作　　者：汪治宇[1] 郭盛虎[1] 李幸[1] 曹静[1] 王聪[2] 韩雪 WANG Zhi -yu , GUO Sheng- hu LI Xing1 , CAO Jing, WANG Cong2, HAN Xue3(1. Department of Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China; 2. Department of Oncology, First Hospital of Hebei Medical University, Shijiazhuang 050011, China; 3. Sports Medical Rehabilitation Center, Hebei Institute of Sports Science, Shijiazhuang 050011, Chin)

机构地区：[1]河北医科大学第四医院肿瘤科,石家庄050011 [2]河北医科大学第一医院肿瘤科,石家庄050011 [3]河北省体育科学研究所运动医疗康复中心,石家庄050011

出　　处：《中国临床药理学杂志》2018年第7期827-830,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省自然科学基金资助项目(H2015206222)

摘　　要：目的研究芍药总苷对6MV-X射线放射性食管炎模型大鼠的影响。方法取健康雄性Wistar大鼠,用6MV-X射线单次照射建立放射性食管炎模型,空白组大鼠17只不接受射线照射。按照体重将造模成功大鼠随机分为4组,每组17只:模型组、对照组和低、高2个剂量实验组。模型组和空白组给予0.9%Na Cl 2 m L,tid;对照组给予西药合剂(0.9%NaCl 250 mL+利多卡因3.2mL+地塞米松1.6 mg+硫酸庆大霉素5.12万单位)2 mL tid;低、高2个剂量实验组分别给予2,6 mg·mL^(-1)芍药总苷溶液2 m L,tid,均连续给药8 d。用光学显微镜观察食管组织病理变化并评分,用酶联免疫吸附法测定血清Ⅲ型前胶原(PCⅢ)、转化生长因子β1(TGF-β1)水平,用蛋白质免疫印迹法检测食管组织神经生长因子(NGF)、酪氨酸蛋白激酶A(Trk A)蛋白、P75蛋白表达水平。结果给药后,空白组、模型组、对照组和低、高2个剂量实验组食管组织病理变化程度评分分别为0,(4.24±0.58),(2.15±0.26),(1.08±0.15),(0.53±0.07)分,这5组的血清PCⅢ水平分别为(40.73±5.24),(90.48±10.38),(71.23±8.72),(58.46±7.66),(49.36±6.12)μg·L^(-1),这5组的食管组织NGF蛋白表达水平分别为0.54±0.07,1.32±0.18,1.13±0.12,0.84±0.11,0.69±0.08,组间比较差异均有统计学意义(均P<0.05)。结论芍药总苷能够减轻6MV-X射线放射性食管炎模型大鼠的病理损伤程度,降低血清PCⅢ、TGF-β1水平,防治食管纤维化,其作用可能与降低NGF及其受体Trk A蛋白和P75蛋白的表达水平有关。Objective To analyze the effect of total glucosides of peony （TGP） on nerve growth factor（NGF） and its receptor in 6MV - X ray ra- diation induced esophagitis model rats. Methods The male Wistar rats were selected, and radiation esophagitis rat model were established by 6MV -X ray single irradiation, the model rats were randomly divided in- to model group, control group, experimental - L group, experimental - H group, each with 17 rats;another 17 rats in blank group did not receive radiation. The model group and blank group were treated with sodium chloride injection 2 mL, tid; the control group was treated with western medicine mixture （ sodium chloride injection 250 mL ± lidocaine 3.2 mL ± dexamethasone 1.6 mg ± gentamicin sulphate 51.2 x 104 u） 2 mL, tid; the experimental -L group and experimental -H group were treatedwith 2,6 mg·m L-1 TGP solution 2 mL,tid. All rats were given the drug continuously for 8 days. The score of patho- logical changes of esophagus were observed by optical microscope. The serum levels of type Ⅲ procollagen （ PC Ⅲ ） and transforming growth factor β1 （TGF - β1 ） were detected by enzyme - linked immunosorbent assay. The expression levels of nerve growth factor（NGF）, tyrosine protein kinase A （TrkA）protein, P75 protein were detected by Western blotting. Results After treatment, the score of pathological changes of esophagus in blank group, model group, control group, experimental - L group, experimental - H group were respectively 0, （4.24 ± 0.58 ）, （ 2. 15 ± 0.26 ） , （ 1.08 ±0. 15 ） and （ 0.53 ± 0.07 ） point, sermn PC 111 levels in the 5 groups were respectively （ 40.73 ± 5.24 ）, （90.48 ± 10.38 ）, （ 71.23 ± 8.72 ）, （ 58.46 ± 7.66 ） and （49.36 ± 6. 12 ） μg·L-1, the expression levels of NGF in esophageal tissue in the 5 groups were respectively 0.54 ± 0. 07, 1.32 ± O. 18, 1.13 ± 0. 12, 0. 84 ± 0. 11 and 0.69 ± 0.08, there were significant differences between groups （ all P 〈 0. 05 ）. Conclusio

关 键 词：芍药总苷 放射性食管炎 神经生长因子 酪氨酸蛋白激酶 

分 类 号：R28[医药卫生—中药学]