枸杞多糖对糖尿病性白内障大鼠晶状体上皮细胞热休克蛋白27表达及氧化应激产物的影响  

Effect of Lycium barbarum polysaccharides on HSP27 expression and oxidative stress products in lens epithelial cells of diabetic cataract rats

作　　者：刘求红[1] 张梅珍 余杨桂[1] 俞晓艺[1] 刘红[1] 程娟娟 LIU Qiu - hong, ZHANG Mei - zhen, YU Yang - gui, YU Xiao - yi, LIU Hong, CHENG Juan - juan(Department of Ophthalmology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Chin)

机构地区：[1]广州中医药大学第一附属医院眼科,广州510405

出　　处：《中国临床药理学杂志》2018年第7期831-833,837,共4页The Chinese Journal of Clinical Pharmacology

基　　金：广东省科技计划基金资助项目(2010B030700070)

摘　　要：目的探讨枸杞多糖对糖尿病性白内障大鼠晶状体上皮细胞热休克蛋白27(HSP27)表达及氧化应激产物的影响。方法选取健康SD大鼠用链脲佐菌素腹腔注射建立糖尿病性白内障大鼠模型。取15只SD健康大鼠予以等量1mol·L^(-1)柠檬酸钠缓冲液,作为对照组。将模型大鼠随机分为模型组、实验A组、实验B组,每组16只。实验A组予以12.5 mg·mL^(-1)氨基胍盐酸盐溶液,每只2 mL;实验B组予以25 mg·m L^(-1)枸杞多糖溶液,每只2 mL;模型组和对照组均予以灭菌0.9%Na Cl,每只2 mL。4组大鼠均予以灌胃给药或0.9%Na Cl,每天1次,连续给药12周。比较4组大鼠的体重、血糖、晶状体混浊相对灰度值、晶状体氧化应激产物指标水平和晶状体上皮细胞HSP27表达水平。结果治疗后,对照组、模型组、实验A组和实验B组的体重分别为(285.64±19.54),(251.73±10.85),(260.89±11.47)和(270.45±13.11)g,血糖分别为(8.12±0.83),(26.47±3.25),(15.18±1.84)和(10.02±0.43)mmo·L^(-1),晶状体混浊相对灰度值分别为(20.28±2.19),(85.66±8.67),(51.18±6.77)和(42.24±5.14),丙二醛分别为(3.95±0.47),(8.15±0.84),(5.73±0.64)和(4.86±0.51)nmol·mg prot^(-1),超氧化物歧化酶分别为(75.74±7.64),(33.26±3.18),(54.07±5.72)和(62.78±8.36)U·mg prot^(-1),谷胱甘肽过氧化物酶分别为(15.78±1.85),(6.25±0.68),(9.88±1.29)和(13.02±1.14)U·mg prot^(-1),HSP27分别为(0.47±0.05),(0.73±0.09),(1.35±0.16)和(1.78±0.22)。模型组、实验A组和实验B组的上述指标与对照组比较,差异均有统计学意义(均P<0.05),且模型组、实验A组和实验B组的上述指标,两两比较,差异均有统计学意义(均P<0.05)。结论枸杞多糖可能通过提高晶状体的抗氧化作用和促进晶状体上皮细胞HSP27表达,来降低糖尿病性白内障大鼠晶状体混浊度,以及改善体重降低和高血糖的情况。Objective To analyze the effect of Lycium barbarum poly- saccharides on heat shock protein 27 （HSP27） expression and oxidative stress products in lens epithelial cells of diabetic cataract rats. Methods Healthy SD rats were selected and diabetic cataract rat models were es- tablished by intraperitoneal injection of streptozotocin. As the control group, another 15 SD healthy rats were given the same amount of 1 mol · L-l sodium citrate buffer. The model rats were randomly divided into model group, test A and B groups with 16 rats per group. Test A group was treated with 12. 5 mg· mL-1 hydrochloride solution, each with2 mL, qd, intragastric administration. Test B group was treated with 25 mg · mL-1 Lycium barbarum polysaccharide solution, each with 2 mL, qd, intragastric administration. Model group and control group were both treated with sterilized 0. 9%NaC1, each with 2 mL, qd, intragastric administration. 4 groups of rats were treated for 12 weeks. The body weight, blood glucose, relative gray value of lens opacity, levels of oxidative stress products indexes and expression level of HSP27 protein in rats of the 4 groups were compared. Results After treatment, the main indexes in control, model, test A and B groups were compared ： weight were （285.64 ± 19.54） , （251.73 ± 10. 85 ） , （ 260. 89 ± 11.47 ） and （270.45 ± 13.11） g, blood glucose were （8.12 ± 0.83）, （26.47 ± 3.25）, （15.18 ± 1.84） and （10.02±0.43） mmo· L-1, relative gray value of lens opacity were （20.28 ± 2. 19）, （85.66 ± 8.67）, （51. 18 ±6. 77） and （42.24 ±5.14）, malondialdehyde were （3.95 ±0.47）, （8.15 ±0. 84）, （5.73 ±0. 64） and （4. 86 ± 0. 51 ） nmol · mg prot - 1, superoxide dismutase were （75.74 ± 7.64）, （33.26 ± 3.18 ）, （54. 07 ± 5.72 ） and （62.78 ±8.36） U. mgprot-1, glutathione peroxidase were （15.78±l.85）, （6. 25 ±0.68）, （9.88 ±1.29） and （13.02 ± 1.14） U · mg prot-1, HSP27 were （0.47 ± 0.05）, （0.73 ± 0.09）,

关 键 词：枸杞多糖 糖尿病性白内障 热休克蛋白27 氧化应激产物 

分 类 号：R28[医药卫生—中药学]