基因重组人生长激素对骨髓基质细胞增殖与成骨性分化的影响  

Effects of recombinant human growth hormone on the proliferation and differentiation of bone marrow stromal cells

作　　者：朱怀安 尹苗 陈婉丽 铁朝荣[1] ZHU Huai-an;YIN Miao;CHEN Wan-li;TIE Chao-rong(Center of stomatology,Zhongnan Hospital, Wuhan Univer- sity, Hubei Wuhan 430071, China)

机构地区：[1]武汉大学中南医院口腔医学中心,湖北武汉430071

出　　处：《临床口腔医学杂志》2018年第3期131-134,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金青年科学基金项目(81500899);湖北省自然科学基金资助项目(220100321)

摘　　要：目的:研究不同浓度基因重组人生长激素(rh GH)作用于体外培养的骨髓基质细胞,对其增殖与成骨性分化能力的影响。方法:取大鼠股骨骨髓,分离骨髓基质细胞进行体外培养,加入不同浓度rh GH(0、2、5、25、50、150、500μg/L),观察细胞增殖率并检测细胞内碱性磷酸酶(ALP)的活性。对细胞进行成骨诱导培养,RT-PCR法检测3种主要成骨标志物(ALP、Co L-1、OCN)的表达水平。结果:不同浓度rh GH对体外培养骨髓基质细胞的生长、ALP活性和相关基因ALP、Co L-l、OCN mRNA表达量均有一定程度促进作用。在150μg/L时对骨髓基质细胞增殖、ALP活性细胞和相关基因ALP、Co L-l、OCN mRNA表达量的促进作用最显著。结论:体外培养rh GH有促进骨髓基质细胞增殖和骨性分化的作用,且rh GH浓度在150μg/L促进作用最显著。Objective：To study the effect of different concentrations of recombinant human growth hormone（rhGH） on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in vitro.Methods：Bone marrow stro-mal cells were isolated from rat bone marrow and cultured in vitro.Different concentrations of rhGH（0,2,5,25,50,150,500 μg/L）were added to observe the cell proliferation rate and detect the activity of intracellular alkaline phosphatase（ALP）. Bone marrow stromal cells were cultured with osteogenesis induced in vitro,and the mRNA expressions of ALP,CoL-1 and OCN were detected by RT-PCR.Results：Different concentrations of rhGH could stimulate the growth of bone marrow stromal cells,enhance the activity of ALP and improve the expression of ALP,Coll,OCN mRNA in vitro.The mRNA expression of ALP,CoL-1,OCN,the activity of ALP and the proliferation rate was significantly increased at 150μg/L.Conclusion：Differ-ent concentrations of rhGH can potentiate the proliferation and differentiation of bone marrow stromal cells in vitro,and it has the most significant effect at the concentration of 150μg/L.

关 键 词：基因重组人生长激素 骨髓基质细胞 骨性分化 

分 类 号：Q813.1[生物学—生物工程]