芪防鼻敏颗粒对变应性鼻炎大鼠炎性因子表达的影响研究  被引量:2

Effect of Qifangbimin Particle on the Expression of Inflammatory Factors in Rats with Allergic Rhinitis

在线阅读下载全文

作  者:孔德明[1] 续艳[1] 杨越 楚慧伦 高琴琴[1] 丁子桐 阎玥[2] 李友林[2] 孙文燕[1] Kong Deming;Xu Yan;Yang Yue;Chu Huilun;Gao Qinqin;Ding Zitong;Yan Yue;Li Youlin;Sun Wenyan(School of Chinese Medica, Beijing University of Chinese Medicine, Beijing 100102, China;Key Institute of State Administration of Traditional Chinese Medicine (Pneumonupathy Chronic Cough and Dyspnea), China-Japan Friendship Hospital)

机构地区:[1]北京中医药大学中药学院,北京100102 [2]中日友好医院国家中医药管理局重点研究室(肺病慢性咳喘)

出  处:《中国药师》2018年第4期593-597,共5页China Pharmacist

基  金:北京市科学技术委员会"十病十药"研发专项课题(编号:Z141100002214018)

摘  要:目的:观察芪防鼻敏颗粒对变应性鼻炎(AR)大鼠血清白介素-4(IL-4),白介素-17(IL-17),肿瘤坏死因子(TNF-α)及鼻黏膜γ干扰素(IFN-γ)、白介素-6(IL-6)、TNF-α表达的影响。方法:SD大鼠随机分为正常组,模型组,氯雷他定组(1.17mg·kg^(-1)),鼻炎康组(0.6 g·kg^(-1)),芪防鼻敏颗粒高(26.48 g·kg^(-1))、中(13.24 g·kg^(-1))、低(6.62 g·kg^(-1))剂量组。采用卵蛋白(OVA)建立AR大鼠模型。造模成功后分别灌胃给药,连续14 d。酶联免疫吸附法(ELISA)检测AR大鼠血清IL-4、IL-17、TNF-α水平,免疫组化染色法(IHC)检测鼻黏膜IFN-γ、IL-6、TNF-α表达。结果:与正常组相比,模型组大鼠血清IL-4、IL-17水平明显上升(P<0.01);鼻黏膜IFN-γ阳性表达明显降低(P<0.05),IL-6、TNF-α阳性表达显著升高(P<0.01)。与模型组相比,氯雷他定组、芪防鼻敏颗粒中剂量组大鼠血清IL-4水平显著下降(P<0.05),芪防鼻敏颗粒低剂量组大鼠血清IL-17水平明显下降(P<0.05),氯雷他定组、鼻炎康组、芪防鼻敏颗粒高、中剂量组大鼠血清TNF-α水平显著下降(P<0.05或P<0.01);高、低剂量组大鼠鼻黏膜IFN-γ阳性表达显著升高(P<0.05或P<0.01),高、中剂量组IL-6阳性表达显著降低(P<0.01),高剂量组TNF-α阳性表达显著降低(P<0.01)。与氯雷他定组相比,鼻炎康组、芪防鼻敏颗粒高剂量组大鼠血清IL-17水平明显上升(P<0.05),鼻炎康组、芪防鼻敏颗粒低剂量组大鼠血清IL-4水平明显上升(P<0.05),芪防鼻敏颗粒高、中、低剂量组TNF-α阳性表达显著升高(P<0.05);与鼻炎康组比较,芪防鼻敏颗粒中剂量组大鼠血清IL-4水平明显下降(P<0.05);与芪防鼻敏颗粒低剂量组相比,芪防鼻敏颗粒高剂量组大鼠血清IL-17水平明显上升(P<0.05),芪防鼻敏颗粒中剂量组大鼠血清IL-4水平明显下降(P<0.05)。结论:芪防鼻敏颗粒可增加AR鼻黏膜IFN-γ表达,降低血清IL-4、TNF-α、IL-17水平及鼻黏膜TNF-α、IL-6表达。Objective: To observe the influence of Qifangbimin particle on the levels of IL-4,IL-17 and TNF-α in serum and the expression of IFN-γ,IL-6 and TNF-α in nasal mucosa of rats with allergic rhinitis( AR). Methods: SD rats were randomly divided into the normal group,model group,loratadine group( 1. 17 mg·kg^(-1)),Biyankang group( 0. 60 g·kg^(-1)),Qifangbimin particle respectively at high dose( 26. 48 g·kg^(-1)),middle dose( 13. 24 g·kg^(-1)) and low dose( 6. 62 g·kg^(-1)) group. AR model was established by ovalbumin( OVA). After the model was successfully made,the rats were administrated with relevant drug continuously for 14 days. The levels of IL-4,IL-17 and TNF-α in serum were measured by enzyme-linked immunosorbent assay( ELISA). The levels of IL-6,IFN-γ and TNF-α in nasal mucosa were detected by immunohistochemical staining( IHC). Results: Compared with those in the normal group,the levels of serum IL-4 and IL-17 significantly increased( P〈0. 01),the optical density value of positive expression of IFN-γ significantly decreased( P〈0. 05),while IL-6 and TNF-α were significantly higher( P〈0. 01) in the nasal mucosa of the model group. Compared with that of the model group,the level of IL-4 in serum was significantly reduced in loratadine group and Qifangbimin particle at middle dose group( P〈0. 05),IL-17 in Qifangbimin particle at low dose group decreased significantly( P〈0. 05),and TNF-α in loratadine group,Biyankang group,Qifangbimin particle at high and middle dose groups significantly decreased( P〈0. 05 or 0. 01); the optical density value of positive expression of IFN-γ significantly increased( P〈0. 05 or 0. 01) in the nasal mucosa of Qifangbimin particle at high and low dose groups,IL-6 in Qifangbimin particle at high and middle dose groups significantly decreased,and TNF-α significantly decreased( P〈0. 05) in Qifangbimin particle at high dose group. Compared with that in loratadine g

关 键 词:芪防鼻敏颗粒 变应性鼻炎 大鼠 细胞因子 

分 类 号:R285.5[医药卫生—中药学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象