正交试验法优选狼毒大戟中活性成分的提取工艺  

Extraction Technology Optimization of the Active Components from Euphorbia Fisheriana by Orthogonal Experiment

作　　者：房俊龙 李文静[1] 洪博[1] Fang Junlong;Li Wenjing;Hong Bo(Pharmacy School, Qiqihar Medical University, Heilongjiang Qiqihar 110016, China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔110016

出　　处：《中国药师》2018年第4期623-625,687,共4页China Pharmacist

基　　金：黑龙江省大学生创新创业项目(编号:201711230032);2017年度黑龙江省留学回国人员择优专项资助项目(编号:2017490);2017年度黑龙江省博士后科研启动基金项目(编号:LBH-Q17179)

摘　　要：目的:优选狼毒大戟中活性成分的最佳提取工艺。方法:选用L_9(3~4)正交试验设计,以提取物中岩大戟內酯B和狼毒乙素的含量为指标,考察乙醇浓度、溶剂用量、提取温度和提取时间对提取效果的影响。结果:最佳提取工艺为料液比1∶10,加入浓度为70%的乙醇溶液,以70℃为提取温度,加热回流提取1 h。结论:优选得到的提取工艺稳定、合理、可行,操作简单,能耗低,大大提高了岩大戟内酯B和狼毒乙素的提取率。Objective： To optimize the extraction technology of active components in Euphorbia fisheriana. Methods： An L_9（ 3~4）orthogonal experiment was performed to investigate the effects of ethanol concentration,solvent amount,extraction temperature and extraction time on the extraction results with the contents of Jolkinolide B and 2,4-dihydroxy-6-methoxy-acetophenone as the evaluation indices. Results： The optimum conditions were as follows： the solid-liquid ratio was 1： 10,70% ethanol was used as the extraction solvent,the extraction temperature was at 70 ℃,and the reflux extraction duration was 1 h. Conclusion： The optimized extraction technology for Jolkinolide B and 2,4-dihydroxy-6-methoxy-acetophenone from Euphorbia fisheriana is stable,reasonable,feasible and easyoperated with low energy consumption,which can greatly enhance the extraction rate of Euphorbia fisheriana.

关 键 词：狼毒大戟 岩大戟内酯B 狼毒乙素 高效液相色谱法 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]