电针对高血脂合并脑缺血大鼠血脂和脑梗死体积的影响及其机制探讨  

Influence of electroacupuncture on blood fat and volume of cerebral infarction and mechanism exploration in rats with hyperlipidemia complicated by cerebral ischemia

作　　者：于若凡 于雪[1] 任秀君[1] Yu Ruofan, Yu Xue, Ren Xiujun(Beijing University of Chinese Medicine, Beijing 100029, Chin)

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2018年第3期227-234,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81470200)~~

摘　　要：目的探讨电针对高血脂合并脑缺血大鼠血脂及脑梗死体积的影响,并初步探讨其作用机制。方法 78只SD大鼠随机分为正常组(各n=6),模型组、假手术组、电针1组、电针2组(n=18)。除正常组外,其余各组喂养高脂饲料6周制备高脂血症模型。6周后电针1组接受电针双侧"丰隆"穴干预,每天1次,连续7 d。第50天,除正常组外,其余各组大鼠建立大脑中动脉血栓闭塞模型。术后电针1、2组均电针"百会"和双侧"丰隆",每天1次。术后1 d检测神经功能和脑梗死体积。术后1、7、14 d检测血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)水平;采用免疫蛋白印迹法检测患侧大脑β-NGF和TrkA蛋白表达。结果术后,模型组TC、TG、LDL升高,HDL降低(P<0.01,P<0.05)。与模型组比较,术后7 d电针1、2组TG降低(P<0.01);术后14 d电针1组LDL降低(P<0.05),电针2组差异无统计学意义。与模型组比较,电针1组大鼠神经功能缺损评分(NDS)降低(P<0.05),电针1、2组大鼠左侧大脑梗死体积减小(P<0.01),且电针1组梗死体积小于电针2组(P<0.05)。术后1 d,与正常组比较,模型组β-神经生长因子(β-NGF)和络氨酸蛋白激酶(TrkA)表达水平有升高趋势,7 d后恢复至正常;与模型组比较,电针1组β-NGF和TrkA表达水平有升高趋势,并且持续升高至术后14 d。结论在高血脂阶段针刺干预,可通过调节血脂水平改善脑缺血后大鼠神经功能,减小脑梗死体积,作用优于脑缺血后针刺治疗。其可能的作用机制在于针刺"丰隆"与"百会"穴促进了β-NGF及其受体TrkA的表达。Objective To discuss the influence of electroacupuncture（ EA） on blood fat and volume of cerebral infarction in rats with hyperlipidemia complicated by cerebral ischemia,and explore the mechanism. Methods SD rats（ n = 78） were randomly divided into normal group（ n = 6）,and model group,sham-operation group,EA group 1 and EA group 2（ each n = 18）. Except of normal group,other groups were given high fat diet for 6 weeks for establishing hyperlipidemia model. After 6 weeks,EA group 1 was given EA in bilateral Fenglong（ ST40） once a day for 7 d. On the 50 thd,the model of middle cerebral artery occlusion（ MCAO） was established in alll groups except of rormal group,and then EA group1 and EA group 2 were given EA in Baihui（ GV20） and bilateral Fenglong（ ST40） once a day. The neurological function and volume of cerebral infarction were detected 1 d after treatment. The levels of total cholesterol（ TC）,triglyceride（ TG）,high-density lipoprotein（ HDL） and low-density lipoprotein（ LDL） were detected 1 d,7 d and 14 d after treatment. The protein expressions of β-NGF and TrkA were detected by using Western blotting assay in diseased brain. Results The levels of TC,G and LDL increased and level of HDL decreased after treatment（ P〈0. 01,P〈0. 05）. Compared with model group,level of TG decreased in EA groups after treatment for 7 d（ P〈0. 01）,and level of LDL decreased in EA group 1 after treatment for 14 d（ P〈0. 05）,which had no significant in EA group 2.Compared with model group,neurological deficit score（ NDS） decreased in EA group 1 after treatment for 1 d（ P〈0. 05）,and left volume of cerebral infarction decreased in EA groups（ P〈0. 01）,which was smaller in EA group 1 than that in EA group 2（ P〈0. 05）. Compared with normal group,expressions of β-NGF and TrkA had an ascending trend in model group after treatment for 1 d,and recover to normal after treatment for 7 d. Compared with model group,expressions of β-NGF and TrkA had an ascendin

关 键 词：电针 高脂血症 脑缺血 Β-神经生长因子 络氨酸蛋白激酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]