水稻葡聚糖内切酶新基因OsGLU16的克隆及特性研究  

Cloning and Characterization Analysis of a New Endoglucanase Gene OsGLU16 in Rice(Oryza sativa L.)

作　　者：朱骞[1,2] 罗樊 张小玲[1] 张慧鑫[1] 汪琨璧 付亚雄 刘思琪 陈丽娟[1,2] 李东宣[1,2] Zhu Qian;Luo Fan;Zhang Xiaoling;Zhang Huixin;Wang Kunbi;Fu Yaxiong;Liu Siqi;Chen Lijuan;Lee Dongsun(Rice Research Institute, Yunnan Agricultural University, Kunming, 650201;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yurman Agricultural University, Kunming, 650201;School of Agricultural Science, Xichang College, Xichang, 615013)

机构地区：[1]云南农业大学稻作研究所,昆明650201 [2]云南农业大学省部共建云南生物资源保护与利用国家重点实验室,昆明650201 [3]西昌学院农业科学学院,西昌615013

出　　处：《分子植物育种》2018年第6期1721-1730,共10页Molecular Plant Breeding

基　　金：本研究由“十三五”国家重点研发计划项目(2016YFD0101101-5)、国家自然科学基金(31560115)、NSFC-云南联合基金重点项目(U11-36604)、省部共建云南生物资源保护与利用国家重点实验室开放课题基金和云南省博士研究生学术新人奖(A3007962)共同资助

摘　　要：β-1,4-葡聚糖内切酶(endo-β-1,4-glucanase,EGase)在植物的组织生长、开花、果实成熟、器官脱落等方面都具有非常重要的作用。本研究克隆了一个新的水稻β-1,4-葡聚糖内切酶家族基因Os GLU16。序列分析表明该基因位于水稻第8号染色体,c DNA全长1 827 bp,包含一个1 572 bp的开放阅读框,编码523个氨基酸残基。蛋白质同源性分析表明,Os GLU16基因编码的蛋白在第54~第516个氨基酸残基区域具有高度保守的GH9催化结构域。本研究成功构建了Os GLU16基因过表达载体p Ca MV35S-Os GLU16-EGFP,并获得了转基因水稻植株。植株表型分析表明,与野生型相比,过表达株系表现为株高和穗长显著下降。本研究初步探明了Os GLU16基因过表达转基因水稻植株的表型特征,这为进一步丰富β-1,4-葡聚糖内切酶基因家族在水稻生长发育过程中的作用提供理论基础。β-1, 4-endoglucanase （endo-β-1, 4-glucanase, EGase） plays an important role in plant tissue growth, flowering, fruit ripening, organ shedding and so on. In this study, we cloned a new gene called OsGLU16 from β-1, 4-glucan endonuclease family in rice. Sequence analysis showed that this gene was located on chromosome 8, and the full length ofcDNA was 1 827 bp. It contained an open reading frame of 1 572 bp, encoding 523 amino acid residues. Protein homology analysis indicated that the protein encoded by the OsGLU16 gene had a highly conserved GH9 catalytic domain in the 54-516 amino acid residue region. We successfully constructed OsGLU16 overexpression vector pCaMV35S-OsGLU16-EGFP and obtained transgenic rice plants. The phenotypic analysis suggested that the overexpression lines had a significant decrease in plant height and spike length compared with wildtype. This study has preliminarily elucidated the phenotypic characteristics of OsGLU16 gene overexpressing transgenic rice plants, which might provide a theoretical basis for further enriching the role of β-1,4-glucan endonuclease gene family in rice growth and development.

关 键 词：β-1 4-葡聚糖内切酶 水稻 OsGLU16 克隆 过表达 

分 类 号：S511[农业科学—作物学]