马铃薯双精氨酸转运系统基因TatA和TatB克隆及功能分析  

Cloning and Functional Analysis of Twin-arginine Translocation Gene TatA and TatB in Potato

作　　者：赵慧 贺引弟 郭江波 辛翠花 Zhao Hui;He Yindi;Guo Jiangbo;Xin Cuihua(School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, 014010)

机构地区：[1]内蒙古科技大学生命科学与技术学院,包头014010

出　　处：《分子植物育种》2018年第6期1746-1751,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31260344;31660414)资助

摘　　要：双精氨酸蛋白转运系统(twin-arginine translocation system,Tat)在伴侣蛋白的校正监控下将已正确折叠的蛋白通过细菌细胞质膜和植物叶绿体类囊体膜。Tat分泌产物的信号序列具典型的双精氨酸基序,包含多拷贝的膜蛋白Tat A、Tat B和Tat C。其中Tat A和Tat B基因在细菌中已进行了大量研究,但是植物中的作用机理尚不清楚。本实验以茄科植物马铃薯为研究对象,克隆基因并采用生物信息学手段进行序列比对,通过烟草脆裂病毒介导的基因沉默技术,结合半定量RT-PCR来解析St Tat A和St Tat B的功能。结果表明:马铃薯St Tat A和St Tat B与大肠杆菌、衣藻、番茄具有高度同源性,且在叶绿体发育过程中起重要作用。The twin-arginine protein translocation system （Tat） transports fold proteins across the bacterial cytoplasmic membrane and the thylakoid membranes of plant chloroplasts under the correction of chaperonin. The signal sequence of TAT-secreted products has the typical Arg-Arg motif, including multi-copy membrane proteins TatA, TatB and TatC. Among them, TarA and TatB genes have been studied extensively in bacteria, but their influence and mechanism in plant have not been clear. In this experiment, potato was selected as the research object. Its genes were cloned and bioinformatics technique was used to carry out sequence alignment. The combination of TRV-induced gene silencing technology and the semi quantitative RT-PCR was applied to reveal the function of StTatA and StTatB. Our results indicated that the StTatA and StTatB from potato had high homology with Escherichia coli, Chlamydomonas reinhardtii and Solanum lycopersicum, and they played an important role in the development of chloroplast as well.

关 键 词：基因StTatA和StTatB 全长克隆 VIGS 半定量RT—PCR 功能分析 

分 类 号：S532[农业科学—作物学]