割手密鸟氨酸转氨酶(Ssδ-OAT-2)基因的克隆与表达分析  

Cloning and Expression Analysis of Ssδ-OAT-2 Gene from Saccharum spontaneum L.

作　　者：姚艳丽[1] 胡小文[1] 徐磊[1] 邢淑莲[1] 高玉尧[1] 安东升[1] 刘洋[1] Yao Yanli;Hu Xiaowen;Xu Lei;Xing Shulian;Gao Yuyao;An Dongsheng;Liu Yang(Guangdong Engineering Technology Research Center for Dryland Water-saving Agriculture, Zhanjiang Experiment Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524013)

机构地区：[1]中国热带农业科学院湛江实验站,广东省旱作节水农业工程技术研究中心,湛江524013

出　　处：《分子植物育种》2018年第6期1812-1817,共6页Molecular Plant Breeding

基　　金：本研究由中国热带农业科学院湛江实验站科研启动基金项目(Zjky201503)和中国热带农业科学院基本科研业务费专项资金项目(1630102016002;17CXTD-07)共同资助

摘　　要：鸟氨酸转氨酶是合成脯氨酸的关键酶之一,在植物适应逆境胁迫中起重要作用。本研究以割手密为研究材料,克隆得到了鸟氨酸转氨酶基因c DNA序列全长,将该基因命名为Ssδ-OAT-2(Gen Bank登陆号:KX714116)。该序列全长1 373 bp,包含一个1 365 bp的开放读码框(ORF),编码454个氨基酸,相对分子质量49.54 k D。同源比对分析表明,Ssδ-OAT-2基因同其近缘属甘蔗的同源性最高(99%),其次是高粱(98%),与其它禾本科植物的同源性约为92%。通过构建与GFP融合表达载体,转洋葱表皮细胞进行瞬时表达,结果表明,该蛋白质主要定位于细胞质和细胞膜上。Ssδ-OAT-2基因的获得为甘蔗抗逆基因工程提供候选基因资源。Ornithine-δ-aminotransferase is considered to be one of the key enzymes for proline biosynthesis, so that it plays an important role in adaptation to environmental stress in plants. Full-length cDNAs of ornithine-δ- aminotransferase, named as Ssδ-OAT-2, were isolated from Saccharum spontaneum using homologous clone strategy. The sequence of this gene was then deposited in GenBank database with the accession number KX714116. Sequence analysis showed that the full length of the Ssδ-OA T-2 gene was 1 373 bp, the open reading frame was 1 365 bp, coding 454 amino acids, and the molecular weight of coded protein was 49.54 kD. The amino acid sequence blast results showed that δ-OAT gene from Saccharum spontaneum shared the highest homology （99%） with relative genera plant Saccharum officinarum, then it had 98% homology with Sorghum bicolor, and had about 92% homology with other grass family. Through the construction of expression vector with GFP fusion, onion epidermal cells did transient expression. The results showed that the protein was mainly located in cytoplasm and cell membrane. The cloning of Ssδ-OA T-2 gene might provide candidate gene resources for resistance gene engineering in sugarcane.

关 键 词：割手密 SsδOAT 基因克隆 亚细胞定位 

分 类 号：S566.1[农业科学—作物学]