利用SLAF-seq技术构建水稻籼粳交DH群体遗传图谱  

Construction of Genetic Map for Rice Doubled Haploid Population by Specific-length Amplified Fragment Sequencing

作　　者：罗曼斯 吴云涛 苏柳 阎新[1] 余潮[1] 李绍波[1] 朱友林[1] 尚俊军[1] Luo Mansi;Wu Yuntao;Su Liu;Yan Xin;Yu Chao;Li Shaobo;Zhu Youlin;Shang Junjun(Key Laboratory of Jiangxi Province for Molecular Biology and Genetic Engineering, College of Life Science in Nanchang University, Nanchang, 330031)

机构地区：[1]南昌大学生命科学学院,江西省分子生物学与基因工程重点实验室,南昌330031

出　　处：《分子植物育种》2018年第6期1923-1928,共6页Molecular Plant Breeding

基　　金：本研究由国家自然科学基金(31660296)和江西省自然科学基金(20142BAB204020)共同资助

摘　　要：我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因组进行比较分析,获得了70 997个在两个亲本中存在多态性的SLAF标签。通过进一步的筛选,我们利用8 940个高质量的SLAF标签构建了该群体的遗传图谱。图谱覆盖12个连锁群,总图距为2 294 71 c M,标记间的平均图距为0.26 c M。遗传图谱的连锁群与水稻物理图的相关系数统计显示,除了9号连锁群的相关系数为0.87外,其余的11个连锁群的相关系数均在0.95以上,表明该图谱的12个连锁群与水稻物理图有很好共线性关系。该高密度图谱的构建为我们进一步研究籼、粳差异基因提供了帮助。A doubled haploid （DH） population constructed by an indica cultivar ZYQ8 and a japonica cultivar JX17 was sequenced by specific-length amplified fragment Sequencing technology. A total of 70 997 SLAF markers were obtained by comparing the sequenced data with rice reference genome. The genetic map for the DH population was constructed by 8 940 selected high quality SLAF markers. The genetic map covered 12 linkage groups and was 2 294 71 cM in length with an average distance of 0.26 cM between adjacent markers. The statistic analysis between the linkage groups of the genetic map and the rice physical map showed that the correlation coefficient of the chromosome 9 was 0.87, and correlation coefficients of the other eleven chromosomes were all above 0.95, indicating a great collinearity relationship between the 12 linkage groups of the genetic map and the rice physical map. The high density genetic map might provide a platform for further study of specific genes in indica and japonica cultivars.

关 键 词：水稻 特定位点扩增片段测序 遗传图谱 

分 类 号：S511[农业科学—作物学]