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作 者:傅巧娟[1] 李春楠[1] 沈国正[1] 陈一[1] 赵福康[1] Fu Qiaojuan;Li Chunnan;Shen Guozheng;Chen Yi;Zhao Fukang(Hangzhou Academy of Agricultural Sciences, Hangzhou, 310024)
出 处:《分子植物育种》2018年第6期2053-2059,共7页Molecular Plant Breeding
基 金:浙江省花卉新品种选育重大科技专项重点项目(2012C12909-12);杭州市科技计划项目(20140932H04)共同资助
摘 要:一串红品种神州红种子用60Coγ射线150 Gy辐射诱变处理,诱变后代材料经多代选择,育成了新品种红运,2016年1月通过浙江省非主要农作物品种审定委员会的审定。与原品种神州红相比,红运的开花期、株型、花序性状及耐热性等均有明显改善。SRAP分析的结果显示,在所用的61对SRAP引物组合中有35对呈现多态性,二者的遗传相似系数为0.960,说明辐射诱变处理引起了一串红遗传物质的变异。本研究的结果证明,辐射诱变对一串红是一种有效的育种途径,SRAP技术是分析辐射诱变的有效方法。A new scarlet sage (Salvia splendens) variety Hongyun was obtained by several generation selections from the progeny of the variety Shenzhouhong carried by ^60Co γ-ray radiation with 150 Gy. Hongyun was approved to release by Non-major Crop Identification Committee of Zhejiang Province in January 2016. Compared with its original variety Shenzhouhong, Hongyun was significantly improved in flowering time, plant shape, inflorescence character and heat resistance trait. SRAP (sequence-related amplified polymorphism) analysis showed that 35 of 61 pairs of primer combinations tested in total were polymorphic and the genetic similarity coefficient between them was 0.960, suggesting that the genetic variation was happened after γ-ray irradiation. Our results could prove that ^60Co γ-ray irradiation was a kind of effective breeding approach for Salvia splendens and the SRAP was an efficient method for analysis of ^60Coγ-ray-induced mutations.
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