早期肠内营养对创伤大鼠β-EP、TNF-α、IL-10的表达及免疫功能的影响  

Effects of early enteral nutrition on the expression of β-EP,TNF-α and IL-10 and the immune function in traumatized rats

作　　者：熊睿[1] 汪晓泊[2] 殷生芝 海应措 张晓雯 朱楠 王小明 XIONG Rui;WANG Xiao-bo;YIN Sheng-zhi;HAI Ying-cuo;ZHANG Xiao-wen;ZHU Nan;WANG Xiao-ming(Department of Clinical Nutrition;Department of Neurology, Qinghai People＇s Hospital, Xining 810007, China)

机构地区：[1]青海省人民医院临床营养科,西宁810007 [2]青海省人民医院神经内科,西宁810007

出　　处：《实用药物与临床》2018年第3期257-260,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的探讨早期肠内营养对创伤大鼠β内啡肽(β-EP)、肿瘤坏死因子α(TNF-α)、白细胞介素10(IL-10)的表达及免疫功能的影响。方法选择10只SD大鼠为A组(正常对照组),30只SD大鼠构建双侧股骨离断创伤大鼠模型,分为B组(创伤对照组)、C组(早期肠内营养组)、D组(延迟肠内营养组),于肠内营养7 d后,分别测量CD3^+、CD4^+、CD8^+水平及β-EP、TNF-α、IL-10水平。结果 B组术后CD4^+细胞数量与CD4^+/CD8^+比值显著低于A组(P<0.05);C组和D组CD4^+与CD4^+/CD8^+比值相对于A组均有下降(P<0.05),但均高于B组(P<0.05),C组CD4^+与CD4^+/CD8^+比值显著高于D组(P<0.05);与A组比较,B组β-EP、TNF-α、IL-10均显著升高(P<0.05)。与B组比较,C组和D组β-EP、TNF-α水平均显著降低,IL-10显著升高(P<0.05)。与D组比较,C组β-EP、TNF-α水平均显著降低,IL-10显著升高(P<0.05)。结论对大鼠实施早期肠内营养可以调节大鼠促、抗炎细胞因子平衡及改善机体免疫功能。Objective To investigate the effects of early enteral nutrition on the expression of beta endorphin（β-EP）,tumor necrosis factor α（TNF-α） and interleukin 10（IL-10） and the immune function in traumatized rats.Methods Totally 10 SD rats were selected as group A（normal control group）;30 SD rats were selected to build the bilateral femoral transection rat models,then they were divided into group B（control group,trauma）,group C（enteral nutrition group）,and group D（delayed enteral nutrition group）;level of CD3 ~＋,CD4 ~＋ and CD8 ~＋ and β-EP,TNF-α,and IL-10 were measured after 7 days’ enteral nutrition.Results The CD4 ~＋ cell number and the CD4 ~＋/CD8 ~＋ ratio of group B were significantly lower than those of group A after the operation（P 〈 0.05）.The CD4 ~＋ and CD4 ~＋/CD8 ~＋ratio of group C and group D were lower than those of group A（P 〈 0.05）,but higher than those of group B（P 〈0.05）;CD4 ~＋ and CD4 ~＋/CD8 ~＋ ratio of group C were significantly higher than those of group D（P 〈 0.05）;the levels of β-EP,TNF-α,and IL-10 of group B were significantly higher than those of group A（P 〈 0.05）.The levels of β-EP and TNF-α of group C and group D were significantly lower than those of group B,and IL-10 was significantly higher than those of group B（P 〈 0.05）.The levels of β-EP and TNF-α of group C were significantly lower than those of group D,and IL-10 was significantly higher than that of group D（P 〈 0.05）.Conclusion Early enteral nutrition can regulate the balance of proinflammatory cytokines and anti-inflammatory cytokines,and can improve the immune function in rats.

关 键 词：早期肠内营养 大鼠 炎性细胞因子 细胞免疫 创伤 

分 类 号：R459.3[医药卫生—治疗学]