双离子对试剂在高效液相色谱法测定盐酸普萘洛尔中的作用  

Role of double ion-pairing agents in determiation of propranolol hydrochloride by HPLC

作　　者：宋辉 高捷 张楠[1] 孟雅 方夏琴 谭晓川 郑稳生[1] SONG Hui;GAO Jie;ZHANG Nan;MENG Ya;FANG Xia-qin;TAN Xiao-chuan;ZHENG Wen-sheng(Institute of Materia Medica, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation, Beijing 100050;Anqing Medical and Pharmaceutical College, Anqing Anhui 246052)

机构地区：[1]北京协和医学院、中国医学科学院药物研究所、药物传输技术及新型制剂北京市重点实验室,北京100050 [2]安庆医药高等专科学校,安徽安庆246052

出　　处：《中南药学》2018年第4期530-532,共3页Central South Pharmacy

摘　　要：目的考察中国药典2015年版盐酸普萘洛尔有关物质检查方法中双离子对试剂对于色谱行为的影响。方法分别调整流动相中十二烷基硫酸钠和四丁基磷酸二氢铵的浓度,更换不同品牌的色谱柱,检查色谱峰保留时间和拖尾因子的变化。结果容量因子的自然对数值与十二烷基硫酸钠摩尔浓度的自然对数值存在近似线性关系;流动相中四丁基磷酸二氢铵浓度增大,拖尾因子降低;不同色谱柱对于相同流动相的反应不同。结论流动相中十二烷基硫酸钠增加盐酸普萘洛尔保留能力,四丁基磷酸二氢胺能起到类似"封端"作用。Objective To determine the effect of double ion-pairing agents on the chromatographic behaviors in examining related substances in Chinese Pharmacopoeia 2015. Methods Changes in retention time and tailing factor were studied with different concentrations of sodium dodecyl sulfate （SDS） and tetrabutylammonium dihydrogen phosphate （TDP）, and columns from different manufacturers. Results The relationship between natural logarithm of capacity factor and natural logarithm of molar concentration of SDS was similarly linear. The tailing factor was reduced with the increase of TDP concentration in the mobile phase. Different chromatographic columns showed different responses to the same mobile phase. Conclusion The propranolol hydrochloride retention capacity increases due to SDS, and TDP plays an ＂end-capping＂ role.

关 键 词：盐酸普萘洛尔 高效液相色谱 双离子对试剂 封端 

分 类 号：O657.63[理学—分析化学]