灰飞虱对杀虫剂的抗性分子机制研究进展  

Research progress in molecular mechanisms of resistance to insecticides in Laodelphax striatellus

作　　者：徐鹿[1] 赵钧[2] 赵春青[2] 徐德进[1] 徐广春[1] 许小龙[1] 张亚楠[3] 张月亮[1] 韩召军[2] 顾中言[1] XU Lu;ZHAO Jun;ZHAO Chunqing;XU Dejin;XU Guangchun;XU Xiaolong;ZHANG Ya＇nan;ZHANG Yueliang;HAN Zhaojun;GU Zhongyan(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanfing 210014, China;College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;College of Life Sciences, Huaibei Normal University, Huaibei 235000, Anhui Province, China)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014 [2]南京农业大学植物保护学院,南京210095 [3]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《农药学学报》2018年第2期135-145,共11页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划(2017YFD0200305);江苏省农业科技自主创新资金项目(CX(16)1001,CX(15)1002)

摘　　要：灰飞虱是中国长江流域和黄淮地区重要的农业害虫,由于杀虫剂的广泛与大量使用,已导致其对多种杀虫剂产生了抗性。深入研究其抗药性分子机制,可为灰飞虱抗性的快速检测和治理提供重要理论基础。文章总结了灰飞虱对毒死蜱、吡虫啉、溴氰菊酯、噻嗪酮、氟虫腈和乙虫腈等杀虫剂的抗性分子机制研究进展,主要包括抗性相关解毒酶和转运蛋白基因的筛选与功能验证,以及靶标位点突变等重要研究成果,指出该研究领域当前存在的问题主要有抗性基因的功能验证及调控路径、抗性新基因的鉴定及交互抗性和多重抗性机制不明确等,并展望了其未来发展方向,认为：可利用CRISPR/Cas9基因编辑技术验证抗性基因功能;可将转录组测序结合生物信息学手段用于鉴定新抗性基因及抗性调控基因,以探明交互抗性和多重抗性机制;应深入至蛋白组学水平探讨抗性机制;需开发配套的高效田间施药技术,以达到杀虫剂减施增效的目的。The small brown planthopper, Laodelphax striatellus（Fallén）, is an important agricultural pest which causes damage to many food crops in Chinese Yangtze River valley and Huanghuai area.The widespread and mass use of insecticides has led to the evolution of resistance to different type insecticides in L. striatellus. Further study on the molecular mechanism of resistance can provide important theoretical basis for the rapid detection and management of resistance of L. striatellus. This review summarized the research progress of the resistance molecular mechanisms, which are induced by chlorpyrifos, imidacloprid, deltamethrin, buprofezin, fipronil and ethiprole, including the identification and functional verification of resistance detoxification enzyme, transport genes and mutations of the target gene site. The current existing problems in this research area mainly focus on the functional validation and regulation path of resistance genes, the identification of new resistance genes, and the unclear mechanism of cross resistance and multiple resistance. Future development directions are expected including functional verification of resistance genes by CRISPR/Cas9 gene editing technology,the identification of new resistance genes and resistance regulation genes by transcriptome sequencing combined with bioinformatics, conformation of cross resistance and multiple resistance mechanism,proteomics level resistance mechanism study, and efficient field spraying technology to reduce pesticides application and improve efficiency of pesticides.

关 键 词：灰飞虱 毒死蜱 溴氰菊酯 噻嗪酮 氟虫腈 抗性分子机制 

分 类 号：S481.4[农业科学—农药学] S482.3[农业科学—植物保护]