附子含药血清对大鼠原代肝细胞能量代谢的影响  

Effect of Serum Containing Aconite on Energy Metabolism of Rat Primary Hepatocytes

作　　者：阚东方 季旭明 陈倩 于婉晨[1] 武继彪 KAN Dongfang;JI Xuming;CHEN Qian;YU Wanchen;WU Jibiao(1. College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China;2. Shandong Medical Technician College, Taian 271000, Shandong, Chin)

机构地区：[1]山东中医药大学中医学院,山东济南250355 [2]山东医药技师学院,山东泰安271000

出　　处：《辽宁中医杂志》2018年第3期645-647,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB512601;2013CB531803);国家自然科学基金面上项目(81673852);山东省重点产业关键技术项目(2016CYS08A01-4,2016CYS08A01-3);山东省重点研发计划计划(2016ZDJS07A12)

摘　　要：目的：探讨附子含药血清对大鼠原代肝细胞能量代谢的影响。方法：制备含药血清,大鼠原代肝细胞随机分为空白血清组和含药血清组。干预24 h后,分光光度计检测细胞内Na~＋-K~＋-ATP酶、Ca^（2＋）-Mg^（2＋）-ATP酶活性,高效液相色谱法（HPLC）检测细胞内ATP、ADP、AMP含量及细胞能荷,激光共聚焦显微镜检测线粒体膜电位。结果：与空白血清相比,附子含药血清可显著升高肝细胞Na~＋-K~＋-ATP酶、Ca^（2＋）-Mg^（2＋）-ATP酶活性,增加细胞内ATP含量,升高线粒体膜电位。结论：热性中药附子促进细胞能量代谢,增加细胞内能量储备。Objective： To observe the effect on energy metabolism of rat primary hepatocytes with serum containing aconite.Methods： Medicated serum was prepared on SD rat. Primary rats hepatocytes were divided into blank serum group and medicated serum group. After intervened for 24 hours,the activities of Na~＋-K~＋-ATPase and Ca^（2＋）-Mg^（2＋）-ATPase were detected with spectrophotometer. The contents of ATP,ADP,AMP were tested with high performance liquid chromatography（ HPLC）. Mitochondrial membrane potential was detected with laser confocal microscope. Results： Compared with blank serum group,serum containing aconite can significantly increase the activities of Na~＋-K~＋-ATPase,Ca^（2＋）-Mg^（2＋）-ATPase and the content of ATP. Mitochondrial membrane potential was also significantly improved. Conclusion： The aconite with heat nature can increase energy reserve and promote energy metabolism.

关 键 词：含药血清 大鼠原代肝细胞 中药药性 能量代谢 

分 类 号：R285[医药卫生—中药学]