大黄素对K562细胞长链非编码RNA表达谱的影响  

Effect of Emodin on Long Non-coding RNA Expression of K562 Cell

作　　者：郑利华[1] 孙士鹏[1] 程实[1] 安成[1] 侯雪筠[1] 叶红蕾 庞博[1] 刘贵建[1] 吴志奎[1] Zheng Lihua, Sun Shipeng, Cheng Shi, et al.(Clinical Laboratories, Guang＇anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, Chin)

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《医学研究杂志》2018年第3期33-36,177,共5页Journal of Medical Research

基　　金：国家重点基础研究发展计划"973"计划项目(2010CB530406);国家自然科学基金青年基金资助项目(81403192)

摘　　要：目的研究大黄素作用K562细胞后长链非编码RNA(lnc RNA)表达谱的变化,筛选并验证差异lnc RNA。方法利用lnc RNA芯片技术检测大黄素作用K562细胞后长链非编码RNA(lnc RNA)表达谱,通过对原始数据进行处理,筛选出差异表达lnc RNA并进行分析,挑选差异较大的4个lnc RNA进行荧光定量PCR验证。结果与DMSO对照组作用K562细胞后lnc RNA表达谱相比,大黄素作用K562细胞后变化4倍以上lnc RNA共11条。经过荧光定量PCR检测,大黄素作用K562细胞后CDR1AS和PROX1-AS1表达上调与芯片结果一致。结论大黄素作用K562细胞后CDR1AS和PROX1-AS1等lnc RNA表达的上调,为进一步深入研究大黄素抑制K562细胞增殖和促进K562细胞红细分化的分子机制打下基础。Objective To analyze the expression profile of long non-coding RNA （lncRNA） in K562 cells after treatment of Emodin. Methods The technique of lncRNA microarray was used to inspect the lncRNA expression profile in K562 cells after treatment of Emodin. The lncRNA was screened out and analyzed by analyzing the original data of lncRNA microarray. Four lncRNAs with large differences were selected for Real-time quantitative PCR analysis. Results 11 lncRNAs were in K562 cells of emodin group which had more than 4-fold variation than DMSO group. The expression of CDR1AS and PROX1-AS1 was up-regulated in K562 cells of emodin group by real-time quantitative PCR analysis. Conclusion Upregulation of CDR1AS and PROX1-AS1 in K562 cells induced by the emodin was the basis for further study of the molecular mechanism of inhibiting K562 cell proliferation and promoting K562 cells to erythropoiesis process.

关 键 词：大黄素 长链非编码RNA CDR1AS PROX1-AS1 

分 类 号：R9[医药卫生—药学]