新型复合敷料促进大鼠Ⅱ°烧伤创面再上皮化的疗效研究  

Research of the Curative Effect about New Compound Dressing in Promoting Epithelial Repaired of Ⅱ° Burned Wound in Rats

作　　者：李百川[1,2] 曹松申 李红丽 Li Baichuan, Cao Songshen, Li Hongli.(Department of Histology and Embryology, The Third Military Medical University, Chongqing 400038, Chin)

机构地区：[1]第三军医大学组织学与胚胎学教研室,重庆400038 [2]中国人民解放军61716部队门诊部,福州350003 [3]中国人民解放军93968部队医院,乌鲁木齐830075

出　　处：《医学研究杂志》2018年第3期105-109,共5页Journal of Medical Research

摘　　要：目的研制一种兼顾抗炎和促进表皮快速再上皮化的新型复合型敷料,并观察其在Ⅱ°烧伤创面中的疗效。方法建立创面损伤模型,将样本随机分成正常、损伤和治疗3个组。治疗组创面在伤后即给予敷料处理。分别于伤后1、3、7、14天测量各组各时期创面的面积,计算创面愈合率。取各组创面皮肤组织,比较各组皮肤组织内总Ca2+浓度及活性氧的差异,并用放射免疫法检测TNF-α、IL-1β及IL-6分泌情况。结果新型复合型敷料明显缩短创面愈合时间,由损伤组29.46±4.38天缩短为25.39±3.12天(P<0.05)。并于伤后14天促进创面皮岛形成,增加愈合率(P<0.05)。复合型敷料能有效降低损伤后创面组织Ca2+浓度,降低炎性因子和ROS水平,两组各时期各指标比较,差异有统计学意义(P<0.05)。结论新型复合敷料可有效加快表皮再上皮化修复,并阻断伤后胞内钙离子浓度升高,减少炎性因子释放,抑制氧化应激反应,为皮肤创面修复提供新的治疗方式。Objective To develop a new compound dressing which can both anti-inflammatory and promote skin quickly epithelial repairing, and then study the curative effect. Methods First, the wound injury model was established. The samples were randomly divided into normal, injury and treatment group which were treated with dressing after injury. Next, the wound skin tissues were taken in injured after 1days, 3days, 7days and 14days. Then, each area of the wound was measured and the wound healing rate were calculated. Total Ca2＋ concentration and the difference of active oxygen in skin tissue between each groups were compared. The secretion of TNF-α, IL-1 and IL-6 were detected by RIA method as follow. Results The new compound dressing can effectively shorten healing time, which is 25.39±3.12days （the treatment group） comparing with 29.46±4.38days （the injury group）（P〈0.05）. And it promotes the formation of skin island in 14days and increase the healing rate（P〈0.05）. Besides, the new compound dressing can effectively reduce the total Ca2＋ concentration, lower the level of the inflammation factors and the ROS after injury, the results of each period of two groups were significant difference（P〈0.05）. Conclusion New compound dressings can effectively promote skin epithelial repairing, block intra-cellular calcium ion channels, reduce the release of inflammation factors and inhibit the reaction of oxidative stress, which provide a new treatment for wound repair.

关 键 词：复合型敷料 钙离子浓度 再上皮化 创面愈合 

分 类 号：R3[医药卫生—基础医学]