姜黄素逆转人胆管癌耐药细胞株QBC939/ADM的初步研究  

A Preliminary Study of QBC939/ADM of Human Bile Duct Cancer Resistant Cell Line by Curcumin

作　　者：刘秀妃 蔺涛 Liu Xiufei;Lin Taoe(Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China;Shanxi University of Traditional Chinese Medicine and Western Medicine Union Hospital, Taiyuan 030013,China)

机构地区：[1]山西中医药大学,山西太原030024 [2]山西中医药大学中西医结合医院,山西太原030013

出　　处：《亚太传统医药》2018年第4期12-14,共3页Asia-Pacific Traditional Medicine

基　　金：山西省中医药管理局科研课题(2016ZYYZ12)

摘　　要：目的:探讨姜黄素(CUR)逆转人胆管癌耐药细胞株QBC939/ADM的耐药作用。方法:以姜黄素作为逆转剂对QBC939/ADM进行干预,采用CCK-8法检测实验组与对照组QBC939/ADM活性,采用流式细胞术检测实验组与对照组QBC939/ADM凋亡率。结果:CCK-8法显示姜黄素分别联合环磷酰胺、5-氟尿嘧啶作用于QBC939/ADM,细胞活性明显低于单独用药,流式细胞仪检测显示姜黄素单独作用于QBC939/ADM细胞凋亡不明显,与环磷酰胺、5-氟尿嘧啶联合使用较单独使用化疗药细胞凋亡率明显增强。结论:姜黄素对QBC939/ADM细胞的多药耐药性有一定的逆转作用,但其具体机制尚未阐明,需进一步探索。Objective： To investigate the drμg resistance of curcumin （CUR） to reverse human cholangiocarcinoma resistant cell line QBC939/ADM. Methods： The QBC939/ADM of QBC939/ADM was interfered with curcumin, and the QBC939/ADM activity, flow cytometry test group and control group QBC939/ADM apoptosis rate were detected by CCK-8 test group and control group. Results： The method of CCK 8 shows curcumin combined cyclophosphamide respectively, in the role of 5-fluorouracil QBC939/ ADM, cell activity significantly lower than the drμgs alone, flow cytometry instrument detection indicated that curcumin separate effect on QBC939/ADM, apoptosis is not obvious, and used in combination with cyclophosphamide, 5 -fluorouracil, a single use, effective cell apoptosis rate significantly increased. Conclusion. Curcumin has a certain reversal effect on the mμLtidrμg resistance of QBC939/ADM cells, but its specific mechanism has not been studied and needs to be further explored.

关 键 词：姜黄素 人胆管癌 QBC939/ADM 多药耐药 

分 类 号：R285.5[医药卫生—中药学]