外源SA与ABA对越冬前后冬小麦‘东农冬麦1号’生理特性的影响  

Winter Wheat ‘Dongnongdongmai 1': Effects of Exogenous SA and ABA on Physiological Characteristics Before and After Overwintering

作　　者：相智也 王秀田[1] 李海丹 张雪霞[1] 张达[1] 闫凯月 张继成 苍晶[1] Xiang Zhiye;Wang Xiutian;Li Haidan;Zhang Xuexia;Zhang Da;Yan Kaiyue;Zhang Jicheng;Cang Jing(School of Life Sciences, Northeast Agricultural University, Harbin 150030)

机构地区：[1]东北农业大学生命科学学院植物生理生化与分子生物学实验室,哈尔滨150030

出　　处：《中国农学通报》2018年第9期8-14,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学人才培养基金科研训练项目"ABA对寒地冬小麦抗寒性的调控及相关抗寒蛋白的分离鉴定"(J1210069);黑龙江省自然科学基金项目"microRNA调控冬小麦东农冬麦1号抗寒性分子机制的研究"(C201418)

摘　　要：本研究以冬小麦品种‘东农冬麦1号’为试验材料,探究了外源SA和ABA对提高冬小麦抗寒性及羿年返青率的影响。三叶期开始用SA、ABA和(ABA+SA)混合液处理麦苗(水作对照CK)。在5℃、0℃、-10℃、-25℃条件下检测麦苗的形态指标、叶绿素荧光特性、生理生化指标及羿年返青率。结果表明:在不同温度下喷施(ABA+SA)均能有效提高麦苗叶片叶绿素含量、Fv/Fm、ΦPSII、qP值而降低NPQ值;随温度的降低,‘DN1’的相对电导率、MDA含量不断增加;喷施SA、ABA、(ABA+SA)与对照组相比,能够不同程度地提高可溶性糖、可溶性蛋白含量以及降低相对电导率和MDA含量,在-10℃条件下(ABA+SA)处理组较其他处理组效果更显著。在0℃条件下(ABA+SA)处理能够显著增加根长和样品干重。ABA和(ABA+SA)处理均能提高返青率,且(ABA+SA)处理的返青率略高。综上,外施(ABA+SA)混合液处理对于提高冬小麦的抗寒性以及提高返青率效果更好。The paper aims to investigate the effects of exogenous SA and ABA on the cold tolerance and regrowth rate of winter wheat‘Dongnongdongmai 1＇. Samples were treated with SA, ABA and a mixture of （ABA ＋ SA） and distilled water as a blank control at the beginning of three-true leaf period; we analyzed morphological index, chlorophyll fluorescence characteristics, physiological and biochemical indicators and regrowth rate of wheat under 5℃, 0℃,-10℃,-25℃. The results showed that： spraying（ABA＋SA） could increase the chlorophyll content at different temperatures and effectively increase the values of Fv/Fm, ΦPSII and qP, but decrease the NPQ; with the decrease of temperature, the relative electrical conductivity, MDA content of ‘DN1＇ increased; compared with the control, SA, ABA and（ABA＋SA） could decrease the relative electricconductivity, MDA content, and increase the content of soluble sugar and soluble protein, and at-10℃,（ABA＋SA） group treatment was more significant than other treatment groups; at 0℃, spraying（ABA ＋ SA） could effectively increase root length and the dry weight of plant; spraying ABA alone and spraying（ABA＋SA） could effectively improve the‘DN1＇regrowth rate, and the regrowth rate of（ABA ＋ SA） treatment was higher.Therefore, spraying（ABA ＋ SA） treatment had better effect on improving cold resistance and reviving rate of winter wheat.

关 键 词：'东农冬麦1号’ SA ABA 越冬前后 生理特性 

分 类 号：Q945[生物学—植物学]