丹皮酚对蛋白聚糖诱导的小鼠关节炎模型骶髂关节滑膜细胞BMP-2、Cbfα1及Smad1mRNA表达的影响  

Effects of Paeonol on Expression of BMP-2, Cbfα1 and Smadl mRNA in Synoviocytes of Sacroiliac Joint of Proteoglycan-Induced Arthritis Model in Mice

作　　者：吴倩[1] 任婕 吴琪 周晓红 杨德才 WU Qian;REN Jie;WU Qi;ZHOU Xiaohong;YANG Decai(The South Courtyard of Shanghai East Hospital, Shanghai 200123;Teaching and Demonstration Center of Clinical Skill, Hubei University of Chinese Medicine, Wuhan 430061;College of Acupuncture and Orthopaedics-Traumatology, Hubei University of Chinese Medicine, Wuhan 430061;The First Clinical College, Hubei University of Chinese Medicine, Wuhan 430061)

机构地区：[1]上海市东方医院南院,上海200123 [2]湖北中医药大学临床技能实训中心,湖北武汉430061 [3]湖北中医药大学针灸骨伤学院,湖北武汉430061 [4]湖北中医药大学第一临床学院,湖北武汉430061

出　　处：《湖北中医药大学学报》2018年第2期18-22,共5页Journal of Hubei University of Chinese Medicine

基　　金：湖北省教育厅科学研究计划资助项目(项目编号:Q20162011)

摘　　要：目的观察丹皮酚对蛋白聚糖诱导的小鼠关节炎模型骶髂关节滑膜细胞BMP-2、Cbfα1及Smad1mRNA表达的影响,初步从分子水平探讨丹皮酚治疗强直性脊柱炎的机理。方法将40只BALB/c小鼠随机分为正常组、模型组、柳氮磺吡啶组和丹皮酚组,每组10只。除正常组外,其余三组采用完全弗氏佐剂+蛋白聚糖腹腔注射建立蛋白聚糖诱导的BALB/c小鼠关节炎模型。实时荧光定量PCR法检测丹皮酚对滑膜细胞BMP-2、Cbfα1及Smad1mRNA表达变化的影响。结果与正常组比较,模型组的BMP-2、Cbfα1和Smad1基因表达明显升高(P<0.01);与模型组比较,柳氮磺吡啶组的BMP-2、Cbfα1和Smad1基因表达差异无统计学意义(P>0.05),而丹皮酚组的基因mRNA表达具有显著差异(P<0.05)。结论丹皮酚能显著抑制强直性脊柱炎病理性骨化,且这种抑制作用与下调滑膜细胞BMP-2、Cbfα1和Smad1基因表达,从而阻断BMP/Smad成骨信号通路有关。Objective To observe the effects of paeonol on the expression of BMP-2, Cbfα1 and Smadl mRNA in synoviocytes of arthritis model in mice induced by proteoglycan, and to explore the mechanism of paeonol in the treatment of ankylosing spondylitis from the molecular level. Methods Forty BALB/c mice were randomly divided into normal group, model group, sulfasalazine group and paeonol group. In addition to normal group, the remaining 3 groups were injected with complete Freund＇s adjuvant and proteoglycan to establish the BALB/c induced proteoglycan arthritis model. The effects of paeonol on the expression of BMP-2, Cbfα1 and Smadl mRNA in synovial cells were detected by real-time fluorescence quantitative PCR. Results Compared with normal group, the expression of BMP-2, Cbfα1 and Smadl in model group was significantly higher （P〈0.01）. Compared with model group, there were no significant differences in the expression of BMP-2, Cbfα1 and Smadl in sulfasalazine group （P〉0.05）, the mRNA expression ofpaeonol group was significantly different （P〈0.05）. Conclusion Paeonol can significantly inhibit the pathological ossification of ankylosing spondylitis, and this inhibition is related to down-regulating the expression of BMP-2, Cbfα1 and Smadl genes in synovial cells, thus blocking the BMP/Smad osteogenesis signal pathway.

关 键 词：实时荧光定量PCR 丹皮酚 BMP-2 Cbfα1 Smad1强直性脊柱炎 

分 类 号：R285.5[医药卫生—中药学]