曲美他嗪对大鼠心肌缺血再灌注后热休克蛋白70及超氧化物歧化酶表达的影响  

Effect of trimetazidine on expression of HSP70 and Mn-SOD after myocardial ischemia/reperfusion in rats

作　　者：钟志英[1] 唐昱[2] 陈图刚[1] 马战清[1] 谭维羚[1] 葛俐娜[1] 刘瑞红 赵宇[1] Zhong Zhiying;Tang Yu;Chen Tugang;Ma Zhanqing;Tan Weiling;Ge Lina;Liu Ruihong;Zhao Yu(Department of Geriatrics, Fourth Affiliated Hospital of Nanchang University, Nanchang 330000 , J iangx＇i Provonce , Chin)

机构地区：[1]南昌大学第四附属医院老年医学科,330000 [2]江西省人民医院心内科

出　　处：《中华老年心脑血管病杂志》2018年第4期408-411,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：江西省卫生计生委资助项目(20155375)

摘　　要：目的探讨缺血再灌注模型大鼠给予曲美他嗪预处理在心肌缺血再灌注保护作用的可能机制。方法选择SD大鼠120只,随机分为对照组、缺血再灌注组、HSP70合成抑制剂组、曲美他嗪组、联合组,每组24只,每组又根据缺血后再灌注时间分为30min、4h及8h3个时间点,每个时间点8只大鼠。每组分别于各时间点测定心肌HSP70阳性细胞数,血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、丙二醛、超氧化物歧化酶(SOD)及心肌梗死面积百分比。结果与对照组比较,缺血再灌注组、HSP70合成抑制剂组、曲美他嗪组、联合组再灌注4及8h血清CK、CK-MB明显升高(P<0.01)。曲美他嗪组再灌注4及8h血清CK、CK-MB较联合组和缺血再灌注组明显降低,再灌注30min、4h及8hHSP70、SOD较联合组和缺血再灌注组明显升高,丙二醛较联合组和缺血再灌注组明显降低(P<0.01)。曲美他嗪组再灌注4及8h心肌梗死面积较联合组和缺血再灌注组明显减少[(39.15±3.62)%vs(44.65±5.14)%、(45.18±3.45)%,P<0.05;(41.28±5.26)%vs(47.54±5.89)%、(46.27±3.94)%,P<0.05]。结论曲美他嗪能升高心肌细胞HSP70水平,减少心肌梗死面积,对缺血再灌注大鼠心肌具有保护作用。Objective To study the protective effect of trimetazidine pretreatment on myocardial I/R in a rat I/R model and its mechanism.Methods One hundred and twenty SD rats were divided into control group,I/R group,HSP70 inhibitor group,trimetazidine treatment group and combined treatment group（24 in each group）.The rats in each group were further divided into 30 min reperfusion group,4 hreperfusion group and 8 hreperfusion group（8 in each group）.Their HSP70-positive myocardial cells were calculated,their serum CK,CK-MB,MDA,SOD levels and their myocardial infarction size were measured.Results The serum CK and CK-MB levels were significantly higher in I/R group,HSP70 inhibitor group,trimetazidine treatment group and combined treatment group than in control group and were significantly lower in trimetazidine treatment group than in combined treatment group and I/R group after reperfusion for 4 and 8 h（P〈0.01）.The serum levels of HSP70 and SOD were significantly higher,the MDA levels were significantly lower in trimetazidine treatment group than in combined treatment group and I/R group after reperfusion for 30 min,4 and 8 h（P〈0.01）.The infraction size was significantly smaller in trimetazidine treatment group than in combined treatment group and I/R group after reperfusion for 4 and 8 h（P〈0.05）.Conclusion Trimetazidine can increase the serum HSP70 level in myocardial cells,reduce the infarction size,and protect the myocardium against I/R in rats.

关 键 词：曲美他嗪 心肌再灌注损伤 HSP70热休克蛋白质类 丙二醛 

分 类 号：R54[医药卫生—心血管疾病]