孕期和哺乳期大鼠甲状腺球蛋白和甲状腺过氧化物酶表达变化的研究  

Expression of thyroglobulin and thyroid peroxidase in pregnant and lactating rats

作　　者：林来祥[1] 孙盟朝 李永梅[1] 乔潇[1] 王帆 梁钰涵 孙毅娜[1] 阎玉芹[1] Lin Laixiang;Sun Mengzhao;Li Yongmei;Qiao Xiao;Wang Fan;Liang Yuhan;Sun Yina;Yan Yuqin(Tianjin Metabolic Diseases Hospital ＆ Tianjin Institute of Endocrinology, Tianjin Medical University, Key Laboratory of Hormones and Development of the National Health and Family Planning Commission, Tianjin Key Laboratory of Metabolic Diseases, Tianjin 300070, China（Lin LX, Li YM, Qiao X, Wang F, Liang YH, Sun YN, Yan YQ;Digestive Department of Tianjin Nankai Hospital, Tianjin 300100, China （Sun MZ)

机构地区：[1]天津医科大学代谢病医院天津市内分泌研究所卫生部激素与发育重点实验室天津市代谢性疾病重点实验室,天津300070 [2]天津南开医院消化内科,天津300100

出　　处：《中华地方病学杂志》2018年第4期287-290,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金（30671816）

摘　　要：目的 探讨孕期和哺乳期大鼠甲状腺球蛋白（Tg）和甲状腺过氧化物酶（TPO）mRNA及蛋白的表达变化。方法 选择SPF／VAF级成年Wistar大鼠96只，其中雌性84只，雄性12只，体质量为220 - 260 g。将雌性大鼠按体质量采用随机数字表法分为7组：对照组，孕早、中、晚期组（7、14、21 d）和哺乳早、中、晚期组（7、14、21 d），每组12只。常规饲料喂养，自由饮用去离子水。除对照组外，将其他6组雌鼠与雄性大鼠按1 ∶ 1合笼交配，分别于孕期和哺乳期7、14、21 d分离甲状腺组织，采用实时荧光定量PCR（qRT-PCR）和蛋白免疫印迹法（Western Blot）检测Tg、TPO的mRNA和蛋白表达。结果 对照组，孕早、中、晚期组和哺乳早、中、晚期组大鼠甲状腺组织的Tg mRNA表达（1.05 ± 0.01、3.20 ± 0.23、1.88 ± 0.12、2.69 ± 0.20、1.53 ± 0.19、2.37 ± 0.31、2.23 ± 0.12）组间比较差异有统计学意义（F = 42.864，P 〈 0.05），孕期和哺乳期各组均高于对照组（P均 〈 0.05）；Tg蛋白的表达（0.15 ± 0.01、0.38 ± 0.01、0.32 ± 0.02、0.37 ± 0.01、0.21 ± 0.01、0.35 ± 0.01、0.44 ± 0.01）组间比较差异有统计学意义（F = 232.250，P 〈 0.05），且孕期和哺乳期各组均高于对照组（P均 〈 0.05）。对照组，孕早、中、晚期组和哺乳早、中、晚期组大鼠甲状腺组织的TPO mRNA的表达（0.57 ± 0.01、0.74 ± 0.03、0.78 ± 0.13、1.08 ± 0.10、0.98 ± 0.10、1.00 ± 0.07、0.76 ± 0.05）组间比较差异有统计学意义（F = 15.448，P 〈 0.05），孕期和哺乳期各组与对照组比较差异有统计学意义（P均 〈 0.05）；TPO蛋白在对照组，孕早、中、晚期组和哺乳早、中、晚期组的表达（0.23 ± 0.01、0.41 ± 0.01、0.72 ± 0.02、0.78 ± 0.01、0.49 ± 0.01、0.52 ± 0.01、0.45 ± 0.02）组间比较差异有统计学意义（F = 563.692，P 〈 0.05），且孕期和哺乳期各组与对照组比较差异均有统计学Objective To observe the mRNA and protein expression of thyroglobulin （Tg） and thyroid peroxidase （TPO） in each trimester of pregnant and lactating Wistar rats. Methods Ninety-six SPF/VAF Wistar rats （84 female and 12 male）, weighting 220 - 260 g were involved. All female Wistar rats were randomly divided into 7 groups according to their body mass via the random number table method： control group, early pregnancy group （7 d）, midpregnancy group （14 d）, late pregnancy group （21 d）, early lactation group （7 d）, midlactation group （14 d） and late lactation group （21 d）, 12 rats in each group. The rats were fed with conventional feed and drank deionized water freely. Female rats of the last 6 groups were mated with male rats. Thyroids were collected on the 7 d, 14 d and 21 d of their pregnancy and lactation, respectively. The mRNA expression levels of Tg and TPO were detected by quantitative real-time PCR, and the protein expression levels of Tg and TPO were detected by Western blotting. Results The expression levels of Tg mRNA in thyroid tissue in the control group, early, middle and late pregnancy and early, middle and late lactation（1.05 ± 0.01, 3.20 ± 0.23, 1.88 ± 0.12, 2.69 ± 0.20, 1.53 ± 0.19, 2.37 ± 0.31, 2.23 ± 0.12） were significantly different between groups （F = 42.864, P 〈 0.05）, and those of pregnancy and lactation groups were higher than those in the control group （P 〈 0.05）. The expression levels of Tg protein were 0.15 ± 0.01, 0.38 ± 0.01, 0.32 ± 0.02, 0.37 ± 0.01, 0.21 ± 0.01, 0.35 ± 0.01, 0.44 ± 0.01, respectively. The differences between groups were statistically significant（F = 232.250, P 〈 0.05）. And those of pregnancy and lactation groups were higher than those in the control group （P 〈 0.05）. The expression levels of TPO mRNA in thyroid tissue in the control group, early, middle and late pregnancy and early, middle and late lactation （0.57 ± 0.01, 0.74 ± 0.03, 0.78 ± 0.13, 1.08 ± 0.10, 0.98 ± 0.10, 1.00 ± 0.

关 键 词：妊娠 动物 哺乳期 大鼠 甲状腺球蛋白 甲状腺过氧化物酶 

分 类 号：R714.7[医药卫生—妇产科学]