NLRP3炎性小体与冠心病的相关性  

The relationship between NLRP3 inflammasome and coronary heart disease

作　　者：胡龙江[1] 周音频[1] 曹运兰 吕湛[3] 蓝运竞[1] 宁琳[1] 向立权[1] 肖鹏[1] HU Long-Jiang;ZHOU Yin-Pin;CAO Yun-Lan;LV Zhan;LAN Yun-Jing;NING Lin;XIANG Li-Quan;XIAO Peng(Department of Cardiology, Fuling Center Hospital of Chongqing City, Chongqing 408000, China;Department of Cardi- ology, Guizhou Provincial People＇s Hospital, Guiyang, Guizhou 550002, China;Department of Cardiology, the Affiliated Hospital of North Sichuan Medical College, Nanchong , Sichuan 637000, China)

机构地区：[1]重庆市涪陵中心医院心血管内科,重庆市408000 [2]贵州省人民医院心血管内科,贵州省贵阳市550002 [3]川北医学院附属医院心血管内科,四川省南充市637000

出　　处：《中国动脉硬化杂志》2018年第3期281-286,共6页Chinese Journal of Arteriosclerosis

基　　金：重庆市卫生和计划生育委员会医学科研项目(2015MSXM138);重庆涪陵区科技计划项目(FLKJ,2014ABB2104)

摘　　要：目的探讨核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎性小体与冠心病（CHD）的临床相关性。方法入选符合纳入标准的冠心病患者60例及对照者30例。收集所有研究对象的性别、年龄、吸烟史、体重指数（BMI）、平均动脉压（MAP）、空腹血糖（FPG）、糖化血红蛋白（Hb A1c）、血脂等临床资料,采用RT-q PCR、Western blot法分别检测各组外周血单个核细胞（PBMC）中NLRP3、凋亡相关微粒蛋白（ASC）、半胱氨酸蛋白酶1（Caspase-1）的mRNA及蛋白表达水平,ELISA法检测各组血浆中白细胞介素1β（IL-1β）、白细胞介素18（IL-18）水平。应用SPSS20.0软件进行统计分析。结果 PBMC中ASC mRNA、NLRP3、ASC、Caspase-1表达水平及血浆IL-1β、IL-18水平在冠心病组明显高于对照组（P〈0.05）。NLRP3、ASC、Caspase-1及其mRNA在各组分别进行Spearman相关分析显示,NLRP3 mRNA和Caspase-1 mRNA在冠心病组均与IL-18呈正相关（r分别为0.327和0.274,P〈0.05）;ASC在冠心病组与年龄、IL-1β、IL-18呈正相关（r分别为0.370、0.467、0.403,P〈0.05）;Caspase-1在冠心病组与吸烟、IL-18呈正相关（r分别为0.613和0.414,P〈0.05）。结论以NLRP3炎性小体为中心的NLRP3-ASC-Caspase-1-IL-1β/IL-18信号通路可能在冠心病的发生发展过程中发挥着重要作用。Aim To investigate the relationship between nucleotide-binding oligomerization domain-like receptor protein 3（ NLRP3） inflammasome and coronary heart disease（ CHD）. Methods 60 CHD patients and 30 controls were recruited according to the inclusion criteria. The clinical data including sex,age,smoking history,body mass index（ BMI）,mean artery pressure（ MAP）,fasting plasma glucose（ FPG）,glycosylated hemoglobin（ Hb A1 c） and blood lipids were collected for analysis. The transcription and expression of NLRP3,apoptosis-associated speck-like protein containing CARD（ ASC） and caspase-1 mRNA as well as protein in peripheral blood mononuclear cells（ PBMC） in all the groups were evaluated by real-time q PCR and Western blot respectively,and the levels of plasma interleukin-1β（ IL-1β） and interleukin-18（ IL-18） in all the groups were detected by ELISA assay. All data were analyzed by SPSS20.0 software.Results The expression levels of the ASC mRNA,NLRP3,ASC,caspase-1 in PBMC and plasma IL-1β,IL-18 in CHD group were significantly higher than those in the control group（ P〈0.05）. The NLRP3,ASC,caspase-1 and their mRNA were evaluated with the Spearman correlation coefficient analysis in each group respectively,both NLRP3 mRNA and caspase-1 mRNA were positively correlated with IL-18 in CHD group,with the correlation coefficients of 0.327（ P = 0.011）and 0.274（ P = 0.031） respectively; ASC were positively correlated with age,IL-18 and IL-1β in CHD group,with the correlation coefficients of 0.370（ P = 0.024）,0.403（ P = 0.027） and 0.467（ P = 0.009） respectively; caspase-1 were positively correlated with smoking and IL-18 in CHD group,with the correlation coefficients of 0. 613（ P = 0. 000） and 0. 414（ P =0.023） respectively. Conclusion NLRP3-ASC-Caspase-1-IL-1β/IL-18 signal pathway centered on the NLRP3 inflammasome may play an important role in the occurrence and development of CHD.

关 键 词：核苷酸结合寡聚化结构域样受体蛋白3 炎性小体 冠心病 白细胞介素18 白细胞介素1Β 

分 类 号：R541.4[医药卫生—心血管疾病]