电针预处理对早发性卵巢功能不全模型大鼠卵巢保护作用的实验研究  

Protective effects of electroacupuncture pretreatment on ovarian in rats with premature ovarian insufficiency

作　　者：王伟明[1] 王扬[1] 吴佳霓[1] 杨丽坤 刘志顺[1] WANG Weiming;WANG Yang;WU Jiani;YANG Likun;LIU Zhishun(Departmentof Acupuncture and Moxibustion, Guang＇anmen Hospital of China Academy of Chinese Medical Sciences, Beijing 100053, China)

机构地区：[1]中国中医科学院广安门医院针灸科,北京100053

出　　处：《中国针灸》2018年第4期405-411,共7页Chinese Acupuncture & Moxibustion

基　　金：中国中医科学院广安门医院所级课题:2016 S 357

摘　　要：目的:探讨电针"中髎""天枢"预处理对早发性卵巢功能不全模型大鼠卵巢的保护作用和可能机制。方法:将动情周期规律的32只SD雌性大鼠随机分为空白组、模型组、电针组、捆绑组,每组8只。模型组、电针组和捆绑组采用去氧乙烯基环己烯(VCD)按160 mg/kg体质量连续腹腔注射15 d造模。电针组自造模之日起于"中髎""天枢"穴进行电针预处理,前2周每日1次、5次/周,后2周隔日1次、3次/周。每次20 min,共4周。捆绑组只捆绑固定,时间、频次和总次数同电针组。空白组、模型组不干预。模型组造模成功后处死大鼠,检测各组大鼠性腺湿重和性腺指数;采用酶联免疫吸附法(ELISA)检测血清性激素抗苗勒激素(AMH)、抑制素B(inhibin B)水平,采用放射免疫分析法检测促卵泡生成素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕酮(P)、睾酮(T)水平,并观察卵巢组织形态和卵泡数量,采用蛋白印迹法检测卵巢组织B淋巴细胞瘤-2基因(Bcl-2)、B淋巴细胞瘤-2相关X蛋白基因(Bax)蛋白表达。结果:与空白组相比,模型组大鼠左、右侧卵巢湿重和卵巢指数均降低,子宫湿重和子宫指数均降低(P<0.05,P<0.01);血清AMH和inhibin B均降低(均P<0.01),血清FSH、LH、T升高(均P<0.01),E2、P降低(均P<0.01);卵巢组织中原始卵泡、初级卵泡相应降低(均P<0.01),闭锁卵泡增多(P<0.01);Bax蛋白表达明显升高(P<0.01),Bcl-2蛋白表达降低(P<0.01)。与模型组相比,电针组大鼠左、右侧卵巢湿重和卵巢指数均升高,子宫湿重升高(P<0.05,P<0.01);血清AMH和inhibin B均升高,血清FSH、LH降低,E2、P升高(P<0.05,P<0.01);卵巢原始卵泡、初级卵泡相应升高,闭锁卵泡减少(P<0.05,P<0.01);卵巢组织Bax蛋白表达降低,Bcl-2蛋白表达升高(P<0.05,P<0.01)。捆绑组和模型组各指标间比较差异均无统计学意义(均P>0.05)。结论:4周的电针"中髎""天枢"预处理对早发性卵巢功能不全模型大鼠的卵巢有一Objective To explore the protective effect and possible mechanism of electroacupuncture（EA） pretreatment at ＂Zhongliao＂（BL 33） and ＂Tianshu＂（ST 25） on ovarian in rats with premature ovarian insufficiency（POI）. Methods A total of 32 SD female rats with regular estrus were randomly divided into a blank group, a model group, an EA group and an immobilization group, 8 rats in each one. The rats in the model group, EA group and immobilization group were treated with intraperitoneal injection of 4-vinylcyclohexene diepoxide（160 mg/kg） for 15 days to establish the model. From the beginning of model establishment, the rats in the EA group were treated with EA pretreatment at ＂Zhongliao＂（BL 33） and ＂Tianshu＂（ST 25）, once a day for the first two weeks（5 treatments per week） and once every two days for the last two weeks（3 treatments per week）. EA was given 20 min per treatment for four weeks. The rats in the immobilization group were treated with immobilization with identical duration, frequency and total amount as the EA group. No treatment was given in the blank group and model group. When model was successfully established in the model group, rats were sacrificed to test the weight of sexual gland and the sexual gland index; the ELISA method was applied to test the level of anti-müllerian hormone（AMH） and inhibin B; the radioimmunoassay method was applied to test the level of follicle-stimulating hormone（FSH）,luteinizing hormone（LH）, estradiol（E2）, progesterone（P） and testosterone（T）. In addition, the morphology and the number of ovarian follicles were observed; and western blot method was applied to test the expression of B-cell lymphoma-2（Bcl-2） and Bcl-2-associated X protein（Bax） in ovarian tissue. Results Compared with the blank group, the bilateral ovary weight, ovary index, uterus weight and uterus index decreased（P〈0.05, P〈0.01）; serum AMH, inhibin B,E2 and P reduced, while serum FSH, LH and T increased in the model group

关 键 词：早发性卵巢功能不全 卵巢保护 电针 穴 中髎 穴 天枢 性激素 

分 类 号：R245.97[医药卫生—针灸推拿学]